Abstract：
Aim: Heparin-induced thrombocytopenia (HIT) is a rare, life-threatening, immune-mediated adverse effect of heparin administration. This study compares frequently used laboratory assays in terms of their effectiveness in HIT diagnosis. Materials & methods: Fifty patients with suspected HIT were tested by gel immunoassay and solid phase PF4/heparin antibody ELISA. On positive results, platelet activation markers P-selectin and Annexin V were assayed using flow cytometry. Results: Thirty/50 patients were negative for both immunoassays. Flow cytometry was performed in the 20 immunoassay positive patients. Platelet activation was observed in 7/20 in the presence of low heparin concentration (0.2 IU/ml). Conclusion: The results are in accordance with the currently available literature and flow cytometry seems a promising alternative in HIT laboratory investigation.
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[Box: see text].
摘要：
目的：肝素诱导的血小板减少症（HIT）是一种罕见的，危及生命,免疫介导的肝素给药的不良反应。本研究比较了常用的实验室检测方法在HIT诊断中的有效性。材料与方法：采用凝胶免疫分析法和固相PF4/肝素抗体ELISA对50例疑似HIT患者进行检测。关于积极的结果，使用流式细胞术测定血小板活化标志物P-选择素和膜联蛋白V。结果：30/50例患者的两种免疫测定均为阴性。对20例免疫测定阳性患者进行流式细胞术。在低肝素浓度（0.2IU/ml）的存在下，在7/20中观察到血小板活化。结论：结果与当前可用的文献一致，流式细胞术在HIT实验室研究中似乎是有希望的替代方法。
[方框：见正文]。
[方框：见正文]。
