{Reference Type}: Journal Article
{Title}: Flow cytometry vs conventional methods for the evaluation of anti-PF4/heparin antibodies: a single center study.
{Author}: Angeli M;Gialeraki A;Anastasopoulou I;Katsarou O;Politou M;
{Journal}: Bioanalysis
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jul 18
{Factor}: 2.695
{DOI}: 10.1080/17576180.2024.2373571
{Abstract}: Aim: Heparin-induced thrombocytopenia (HIT) is a rare, life-threatening, immune-mediated adverse effect of heparin administration. This study compares frequently used laboratory assays in terms of their effectiveness in HIT diagnosis. Materials & methods: Fifty patients with suspected HIT were tested by gel immunoassay and solid phase PF4/heparin antibody ELISA. On positive results, platelet activation markers P-selectin and Annexin V were assayed using flow cytometry. Results: Thirty/50 patients were negative for both immunoassays. Flow cytometry was performed in the 20 immunoassay positive patients. Platelet activation was observed in 7/20 in the presence of low heparin concentration (0.2 IU/ml). Conclusion: The results are in accordance with the currently available literature and flow cytometry seems a promising alternative in HIT laboratory investigation.<BR>[Box: see text].