PDF(Pubmed)
Abstract：
In Escherichia coli, it is debated whether the two replisomes move independently along the two chromosome arms during replication or if they remain spatially confined. Here, we use high-throughput fluorescence microscopy to simultaneously determine the location and short-time-scale (1 s) movement of the replisome and a chromosomal locus throughout the cell cycle. The assay is performed for several loci. We find that (i) the two replisomes are confined to a region of ~250 nm and ~120 nm along the cell\'s long and short axis, respectively, (ii) the chromosomal loci move to and through this region sequentially based on their distance from the origin of replication, and (iii) when a locus is being replicated, its short time-scale movement slows down. This behavior is the same at different growth rates. In conclusion, our data supports a model with DNA moving towards spatially confined replisomes at replication.
摘要：
在大肠杆菌中，在复制过程中，两个复制体是否沿着两个染色体臂独立移动，或者它们是否保持空间受限，存在争议。这里,我们使用高通量荧光显微镜在整个细胞周期中同时确定复制体和染色体位点的位置和短时间尺度(1s)运动.对几个基因座进行测定。我们发现（i）两个复制体沿着细胞的长轴和短轴被限制在〜250nm和〜120nm的区域，分别,（ii）染色体基因座根据其与复制起点的距离依次移动并通过该区域，和（iii）当复制基因座时，它的短时间尺度运动减慢。这种行为在不同的增长率下是相同的。总之,我们的数据支持复制时DNA向空间受限的复制体移动的模型.
