Abstract：
Cocaine (COC) and benzoylecgonine (BE), the main COC metabolite, have been detected in aquatic ecosystems. Studies focusing on wild fish are, however, very limited, and no reports concerning elasmobranchs are available. This study investigated COC and BE levels in Brazilian Sharpnose sharks (Rhizoprionodon lalandii) (n = 13) using LC-MS/MS. All samples (13/13) tested positive for COC, with 92 % (12/13) testing positive for BE. COC concentrations (23.0 μg kg-1) were over 3-fold higher than BE (7.0 μg kg-1). COC levels were about three-fold significantly higher in muscle (33.8 ± 33.4 g kg-1) compared to liver (12.2 ± 14.2 μg kg-1). Females presented higher COC concentrations in muscle (40.2 ± 35.8 μg kg-1) compared to males (12.4 ± 5.9 μg kg-1). Several positive statistical correlations were noted between COC and BE (rho = 0.84) in females, indicating systemic COC transport and metabolization, as well as between BE and weight (rho = 0.62), and between COC and the Condition Factor (rho = 0.73). A strong correlation was noted between BE and COC in the muscle of non-pregnant females (rho = 1.00). This study represents the first COC and BE report in free-ranging sharks, and the findings point to the potential impacts of the presence of illicit drugs in environments.
摘要：
可卡因（COC）和苯甲酰芽子碱（BE），主要的COC代谢产物，已在水生生态系统中检测到。针对野生鱼类的研究是，然而,非常有限,并且没有关于弹性膜的报告。本研究使用LC-MS/MS调查了巴西鲨鱼（Rhizoprionodonlalandii）（n=13）的COC和BE水平。所有样本（13/13）的COC检测呈阳性，92%(12/13)的BE检测呈阳性。COC浓度（23.0μgkg-1）比BE（7.0μgkg-1）高3倍以上。与肝脏（12.2±14.2μgkg-1）相比，肌肉（33.8±33.4gkg-1）中的COC水平显着高出约三倍。与男性（12.4±5.9μgkg-1）相比，女性在肌肉中的COC浓度更高（40.2±35.8μgkg-1）。在女性中，COC和BE之间存在一些正的统计相关性（rho=0.84）。指示系统性COC运输和代谢，以及介于BE和体重之间(rho=0.62)，在COC和条件因子之间(rho=0.73)。在非妊娠女性的肌肉中注意到BE和COC之间的强相关性(rho=1.00)。这项研究代表了自由放养鲨鱼的第一份COC和BE报告，研究结果指出了环境中存在非法药物的潜在影响。
