PDF(Pubmed)
Abstract：
This experiment was conducted to investigate the effects of mulberry leaf extract (MLE) on alleviating fatty liver hemorrhagic syndrome (FLHS) in laying hens. The 576 Jing Fen laying hens of 56 weeks of age with good health and similar weights (1.76 ± 0.17 kg) were randomly divided into 6 groups, with 8 replicates in each group and 12 chickens in each replicate. The experiment lasted 56 d. The control group was fed a corn-soybean meal diet. The FLHS group was fed a high energy-low protein (HELP) diet, and the other four experimental groups were fed HELP diets supplemented with 0.04, 0.40, 0.80, and 1.20% MLE, respectively. The results showed that HELP treatment significantly induced liver injury, which indicated that the FLHS model was successfully established. MLE supplementation could alleviate the FLHS by reducing the liver index, abdominal fat percentage, total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) in the serum (P < 0.05), and subsequently increase the egg production rate (P < 0.05). The laying hens fed 0.8% MLE exhibited the greatest production performance (P < 0.05) and could improve serum lipid levels. In addition, the genes associated with fatty acid synthesis (ACC, HMGR and SREBP-1C) were downregulated (P < 0.05), and genes related to fatty acid oxidation (CPT1A, AMPK, and ATGL) were found to be upregulated (P < 0.05). Supplementation with 1.2% MLE significantly reduced the relative abundance of Firmicutes and Desulfurized Bacillus (P < 0.05) and significantly increased the relative abundance of Fecal Bacillus (P < 0.05). In conclusion, MLE may regulate the mRNA expression of lipid metabolism-related genes through the AMPK signaling pathway and improve cecal microbiota balance and serum lipid levels to alleviate FLHS in laying hens and subsequently improve egg production performance.
摘要：
本试验旨在研究桑叶提取物(MLE)对蛋鸡脂肪肝出血性综合征(FLHS)的缓解作用。将576只56周龄健康、体重相近(1.76±0.17kg)的静粉蛋鸡随机分为6组,每组重复8个，每个重复12只鸡。实验持续56d。对照组饲喂玉米豆粕日粮。FLHS组饲喂高能量低蛋白（HELP）饮食，其他四个实验组饲喂添加0.04、0.40、0.80和1.20%MLE的HELP日粮，分别。结果表明，HELP治疗显著诱导肝损伤，表明FLHS模型成功建立。补充MLE可以通过降低肝脏指数来缓解FLHS，腹部脂肪百分比，总胆固醇(TC),甘油三酯（TG），低密度脂蛋白（LDL），和极低密度脂蛋白（VLDL）在血清中（P<0.05），提高产蛋率(P<0.05)。饲喂0.8％MLE的蛋鸡表现出最大的生产性能（P<0.05），并且可以改善血清脂质水平。此外,与脂肪酸合成相关的基因(ACC，HMGR和SREBP-1C)表达下调(P<0.05),和与脂肪酸氧化相关的基因(CPT1A，AMPK,和ATGL)被发现上调(P<0.05)。添加1.2%MLE可显著降低厚壁菌和脱硫芽孢杆菌的相对丰度(P<0.05),显著提高粪便芽孢杆菌的相对丰度(P<0.05)。总之,MLE可能通过AMPK信号通路调节脂质代谢相关基因的mRNA表达，改善盲肠微生物平衡和血脂水平，从而缓解蛋鸡的FLHS，进而提高产蛋性能。
