Abstract：
Humus (HS) reservoirs can embed microbial necromass (including cell wall components that are intact or with varying degrees of fragmentation) in small pores, raising widespread concerns about the potential for C/N interception and stability in composting systems. In this study, fresh cow manure and sawdust were used for microbial solid fermentation, and the significance of microbial residues in promoting humification was elucidated by measuring their physicochemical properties and analyzing their microbial informatics. These results showed that the stimulation of external carbon sources (NaHCO3) led to an increase in the accumulation of bacterial necromass C/N from 6.19 and 0.91 µg/mg to 21.57 and 3.20 µg/mg, respectively. Additionally, fungal necromass C/N values were about 3 times higher than the initial values. This contributed to the increase in HS content and the increased condensation of polysaccharides and nitrogen-containing compounds during maturation. The formation of cellular debris mainly depends on the enrichment of Actinobacteria, Proteobacteria, Ascomycota, and Chytridiomycota. Furthermore, Euryarchaeota was the core functional microorganism secreting cell wall lytic enzymes (including AA3, AA7, GH23, and GH15). In conclusion, this study comprehensively analyzed the transformation mechanisms of cellular residuals at different profile scales, providing new insights into C/N cycles and sequestration.
摘要：
腐殖质（HS）储层可以在小孔中嵌入微生物坏死（包括完整或具有不同程度的破碎的细胞壁成分），引起了人们对堆肥系统中C/N截留和稳定性的潜在担忧。在这项研究中,新鲜牛粪和木屑用于微生物固体发酵，通过测定微生物残留物的理化性质和分析其微生物信息学，阐明了微生物残留物在促进腐殖化中的意义。这些结果表明，外部碳源（NaHCO3）的刺激导致细菌坏死C/N的积累从6.19和0.91µg/mg增加到21.57和3.20µg/mg，分别。此外,真菌坏死的C/N值比初始值高约3倍。这有助于HS含量的增加以及成熟过程中多糖和含氮化合物的缩合增加。细胞碎片的形成主要依赖于放线菌的富集,变形杆菌,子囊,和衣原体.此外，欧洲古细菌是分泌细胞壁裂解酶（包括AA3，AA7，GH23和GH15）的核心功能微生物。总之,本研究全面分析了细胞残余物在不同剖面尺度下的转化机制，提供对C/N循环和隔离的新见解。
