PDF(Pubmed)
Abstract：
UNASSIGNED: The present study investigated the nephron-testicular protective effects of sesamin against cisplatin (CP)-induced acute renal and testicular injuries.
UNASSIGNED: Thirty-two male Wistar rats were allocated to receive carboxymethylcellulose (0.5%, as sesamin vehicle), CP (a single i.p. 5 mg/kg dose), CP plus sesamin at 10 or 20 mg/kg orally for 10 days.
UNASSIGNED: Data analysis showed significant increases in serum urea, creatinine, interleukin (IL)-1, IL-6, and tumor necrosis factor-α (TNF-α), as well as renal and testicular tissue malondialdehyde and nitric-oxide concentrations in CP-intoxicated rats in comparison to control animals. On the contrary, rats treated with CP only exhibited significantly lower (p < .05) serum testosterone, tissue glutathione, and activities of endogenous antioxidant enzymes compared to control rats. Histopathologically examining CP-intoxicated rats\' tissues using H&E and PAS stains showed atrophied glomeruli, interstitial inflammatory cells, atypic tubular epithelium with focal apoptosis, and reduced mucopolysaccharide content. Further, immunohistochemical staining of the same group revealed an increase in p53 and cyclooxygenase-II (Cox-II) expression in renal and testicular tissues. Treatment with sesamin alleviated almost all the changes mentioned above in a dose-dependent manner, with the 20 mg/kg dose restoring several parameters\' concentrations to normal ranges.
UNASSIGNED: In brief, sesamin could protect the kidneys and testes against CP toxicity through its antioxidant, anti-inflammatory, and anti-apoptotic effects.
UNASSIGNED: Thirty-two male Wistar rats were allocated to receive carboxymethylcellulose (0.5%, as sesamin vehicle), CP (a single i.p. 5 mg/kg dose), CP plus sesamin at 10 or 20 mg/kg orally for 10 days.
UNASSIGNED: Data analysis showed significant increases in serum urea, creatinine, interleukin (IL)-1, IL-6, and tumor necrosis factor-α (TNF-α), as well as renal and testicular tissue malondialdehyde and nitric-oxide concentrations in CP-intoxicated rats in comparison to control animals. On the contrary, rats treated with CP only exhibited significantly lower (p < .05) serum testosterone, tissue glutathione, and activities of endogenous antioxidant enzymes compared to control rats. Histopathologically examining CP-intoxicated rats\' tissues using H&E and PAS stains showed atrophied glomeruli, interstitial inflammatory cells, atypic tubular epithelium with focal apoptosis, and reduced mucopolysaccharide content. Further, immunohistochemical staining of the same group revealed an increase in p53 and cyclooxygenase-II (Cox-II) expression in renal and testicular tissues. Treatment with sesamin alleviated almost all the changes mentioned above in a dose-dependent manner, with the 20 mg/kg dose restoring several parameters\' concentrations to normal ranges.
UNASSIGNED: In brief, sesamin could protect the kidneys and testes against CP toxicity through its antioxidant, anti-inflammatory, and anti-apoptotic effects.
摘要：
■本研究调查了芝麻素对顺铂（CP）引起的急性肾和睾丸损伤的肾睾丸保护作用。
■32只雄性Wistar大鼠被分配接受羧甲基纤维素（0.5％，作为芝麻素车辆)，CP（单次腹膜内注射5mg/kg剂量），CP加芝麻素以10或20mg/kg口服10天。
■数据分析显示血清尿素显著增加，肌酐,白细胞介素（IL）-1，IL-6和肿瘤坏死因子-α（TNF-α），与对照动物相比，CP中毒大鼠的肾脏和睾丸组织丙二醛和一氧化氮浓度。相反,用CP处理的大鼠仅表现出显著降低(p<0.05)的血清睾酮,组织谷胱甘肽，与对照大鼠相比，内源性抗氧化酶的活性。使用H&E和PAS染色对CP中毒大鼠组织进行组织病理学检查，显示肾小球萎缩，间质炎症细胞，异型肾小管上皮细胞局灶性凋亡，和减少粘多糖含量。Further,同一组的免疫组织化学染色显示肾和睾丸组织中p53和环氧合酶II（Cox-II）表达增加。芝麻素治疗以剂量依赖性方式缓解了上述几乎所有的变化，20mg/kg剂量将几个参数\'浓度恢复到正常范围。
■简而言之，芝麻素可以通过其抗氧化剂保护肾脏和睾丸免受CP毒性，抗炎,和抗凋亡作用。
■32只雄性Wistar大鼠被分配接受羧甲基纤维素（0.5％，作为芝麻素车辆)，CP（单次腹膜内注射5mg/kg剂量），CP加芝麻素以10或20mg/kg口服10天。
■数据分析显示血清尿素显著增加，肌酐,白细胞介素（IL）-1，IL-6和肿瘤坏死因子-α（TNF-α），与对照动物相比，CP中毒大鼠的肾脏和睾丸组织丙二醛和一氧化氮浓度。相反,用CP处理的大鼠仅表现出显著降低(p<0.05)的血清睾酮,组织谷胱甘肽，与对照大鼠相比，内源性抗氧化酶的活性。使用H&E和PAS染色对CP中毒大鼠组织进行组织病理学检查，显示肾小球萎缩，间质炎症细胞，异型肾小管上皮细胞局灶性凋亡，和减少粘多糖含量。Further,同一组的免疫组织化学染色显示肾和睾丸组织中p53和环氧合酶II（Cox-II）表达增加。芝麻素治疗以剂量依赖性方式缓解了上述几乎所有的变化，20mg/kg剂量将几个参数\'浓度恢复到正常范围。
■简而言之，芝麻素可以通过其抗氧化剂保护肾脏和睾丸免受CP毒性，抗炎,和抗凋亡作用。
