PDF(Pubmed)
Abstract：
BACKGROUND: Chagas disease (CD), a neglected parasitic disease caused by Trypanosoma cruzi, poses a significant health threat in Latin America and has emerged globally because of human migration. Trypanosoma cruzi infects humans and over 100 other mammalian species, including dogs, which are important sentinels for assessing the risk of human infection. Nonetheless, the serodiagnosis of T. cruzi in dogs is still impaired by the absence of commercial tests. In this study, we investigated the diagnostic accuracy of four chimeric recombinant T. cruzi IBMP antigens (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) for detecting anti-T. cruzi antibodies in dogs, using latent class analysis (LCA).
METHODS: We examined 663 canine serum samples, employing indirect ELISA with the chimeric antigens. LCA was utilized to establish a latent variable as a gold standard for T. cruzi infection, revealing distinct response patterns for each antigen.
RESULTS: The IBMP (Portuguese acronym for the Molecular Biology Institute of Paraná) antigens achieved area under the ROC curve (AUC) values ranging from 90.9% to 97.3%. The highest sensitivity was attributed to IBMP-8.2 (89.8%), while IBMP-8.1, IBMP-8.3, and IBMP-8.4 achieved 73.5%, 79.6%, and 85.7%, respectively. The highest specificity was observed for IBMP-8.4 (98.6%), followed by IBMP-8.2, IBMP-8.3, and IBMP-8.1 with specificities of 98.3%, 94.4%, and 92.7%, respectively. Predictive values varied according to prevalence, indicating higher effectiveness in endemic settings.
CONCLUSIONS: Our findings underscore the remarkable diagnostic performance of IBMP-8.2 and IBMP-8.4 for the serodiagnosis of Trypanosoma cruzi in dogs, representing a promising tool for the diagnosis of CD in dogs. These chimeric recombinant antigens may not only enhance CD surveillance strategies but also hold broader implications for public health, contributing to the global fight against this neglected tropical disease.
METHODS: We examined 663 canine serum samples, employing indirect ELISA with the chimeric antigens. LCA was utilized to establish a latent variable as a gold standard for T. cruzi infection, revealing distinct response patterns for each antigen.
RESULTS: The IBMP (Portuguese acronym for the Molecular Biology Institute of Paraná) antigens achieved area under the ROC curve (AUC) values ranging from 90.9% to 97.3%. The highest sensitivity was attributed to IBMP-8.2 (89.8%), while IBMP-8.1, IBMP-8.3, and IBMP-8.4 achieved 73.5%, 79.6%, and 85.7%, respectively. The highest specificity was observed for IBMP-8.4 (98.6%), followed by IBMP-8.2, IBMP-8.3, and IBMP-8.1 with specificities of 98.3%, 94.4%, and 92.7%, respectively. Predictive values varied according to prevalence, indicating higher effectiveness in endemic settings.
CONCLUSIONS: Our findings underscore the remarkable diagnostic performance of IBMP-8.2 and IBMP-8.4 for the serodiagnosis of Trypanosoma cruzi in dogs, representing a promising tool for the diagnosis of CD in dogs. These chimeric recombinant antigens may not only enhance CD surveillance strategies but also hold broader implications for public health, contributing to the global fight against this neglected tropical disease.
摘要：
背景：查加斯病（CD），一种被忽视的由克氏锥虫引起的寄生虫病，在拉丁美洲构成了重大的健康威胁，并且由于人类迁移而在全球范围内出现。克氏锥虫感染人类和其他100多种哺乳动物，包括狗,这是评估人类感染风险的重要哨兵。尽管如此，由于缺乏商业测试，犬中克氏杆菌的血清诊断仍然受到损害。在这项研究中,我们调查了四种嵌合重组克氏T.cruziIBMP抗原（IBMP-8.1，IBMP-8.2，IBMP-8.3和IBMP-8.4）用于检测抗T.狗的克鲁氏抗体，使用潜在类分析(LCA)。
方法：我们检测了663份犬血清样本，采用间接ELISA与嵌合抗原。LCA被用来建立一个潜在变量作为克氏虫感染的黄金标准，揭示每种抗原的不同反应模式。
结果：IBMP（巴拉那州分子生物学研究所的葡萄牙语缩写）抗原的ROC曲线下面积（AUC）值为90.9％至97.3％。最高的灵敏度归因于IBMP-8.2（89.8％），而IBMP-8.1、IBMP-8.3和IBMP-8.4达到73.5%，79.6%,和85.7%，分别。观察到最高的特异性为IBMP-8.4（98.6%），其次是IBMP-8.2、IBMP-8.3和IBMP-8.1，特异性为98.3%，94.4%,92.7%,分别。预测值根据患病率的不同而不同，表明在地方性环境中的有效性更高。
结论：我们的发现强调了IBMP-8.2和IBMP-8.4在犬中对克氏锥虫的血清诊断的显着诊断性能，代表了狗CD诊断的一个有前途的工具。这些嵌合重组抗原不仅可以增强CD监测策略，而且对公共卫生具有更广泛的影响。为全球抗击这种被忽视的热带病做出贡献。
方法：我们检测了663份犬血清样本，采用间接ELISA与嵌合抗原。LCA被用来建立一个潜在变量作为克氏虫感染的黄金标准，揭示每种抗原的不同反应模式。
结果：IBMP（巴拉那州分子生物学研究所的葡萄牙语缩写）抗原的ROC曲线下面积（AUC）值为90.9％至97.3％。最高的灵敏度归因于IBMP-8.2（89.8％），而IBMP-8.1、IBMP-8.3和IBMP-8.4达到73.5%，79.6%,和85.7%，分别。观察到最高的特异性为IBMP-8.4（98.6%），其次是IBMP-8.2、IBMP-8.3和IBMP-8.1，特异性为98.3%，94.4%,92.7%,分别。预测值根据患病率的不同而不同，表明在地方性环境中的有效性更高。
结论：我们的发现强调了IBMP-8.2和IBMP-8.4在犬中对克氏锥虫的血清诊断的显着诊断性能，代表了狗CD诊断的一个有前途的工具。这些嵌合重组抗原不仅可以增强CD监测策略，而且对公共卫生具有更广泛的影响。为全球抗击这种被忽视的热带病做出贡献。
