METHODS: We examined 663 canine serum samples, employing indirect ELISA with the chimeric antigens. LCA was utilized to establish a latent variable as a gold standard for T. cruzi infection, revealing distinct response patterns for each antigen.
RESULTS: The IBMP (Portuguese acronym for the Molecular Biology Institute of Paraná) antigens achieved area under the ROC curve (AUC) values ranging from 90.9% to 97.3%. The highest sensitivity was attributed to IBMP-8.2 (89.8%), while IBMP-8.1, IBMP-8.3, and IBMP-8.4 achieved 73.5%, 79.6%, and 85.7%, respectively. The highest specificity was observed for IBMP-8.4 (98.6%), followed by IBMP-8.2, IBMP-8.3, and IBMP-8.1 with specificities of 98.3%, 94.4%, and 92.7%, respectively. Predictive values varied according to prevalence, indicating higher effectiveness in endemic settings.
CONCLUSIONS: Our findings underscore the remarkable diagnostic performance of IBMP-8.2 and IBMP-8.4 for the serodiagnosis of Trypanosoma cruzi in dogs, representing a promising tool for the diagnosis of CD in dogs. These chimeric recombinant antigens may not only enhance CD surveillance strategies but also hold broader implications for public health, contributing to the global fight against this neglected tropical disease.
方法:我们检测了663份犬血清样本,采用间接ELISA与嵌合抗原。LCA被用来建立一个潜在变量作为克氏虫感染的黄金标准,揭示每种抗原的不同反应模式。
结果:IBMP(巴拉那州分子生物学研究所的葡萄牙语缩写)抗原的ROC曲线下面积(AUC)值为90.9%至97.3%。最高的灵敏度归因于IBMP-8.2(89.8%),而IBMP-8.1、IBMP-8.3和IBMP-8.4达到73.5%,79.6%,和85.7%,分别。观察到最高的特异性为IBMP-8.4(98.6%),其次是IBMP-8.2、IBMP-8.3和IBMP-8.1,特异性为98.3%,94.4%,92.7%,分别。预测值根据患病率的不同而不同,表明在地方性环境中的有效性更高。
结论:我们的发现强调了IBMP-8.2和IBMP-8.4在犬中对克氏锥虫的血清诊断的显着诊断性能,代表了狗CD诊断的一个有前途的工具。这些嵌合重组抗原不仅可以增强CD监测策略,而且对公共卫生具有更广泛的影响。为全球抗击这种被忽视的热带病做出贡献。