PDF(Pubmed)
Abstract：
This study delves into the genomic features of 10 Vibrio strains collected from deep-sea hydrothermal vents in the Pacific Ocean, providing insights into their evolutionary history and ecological adaptations. Through sequencing and pan-genome analysis involving 141 Vibrio species, we found that deep-sea strains exhibit larger genomes with unique gene distributions, suggesting adaptation to the vent environment. The phylogenomic reconstruction of the investigated isolates revealed the presence of 2 main clades: The first is monophyletic, consisting exclusively of Vibrio alginolyticus, while the second forms a monophyletic clade comprising both Vibrio antiquarius and Vibrio diabolicus species, which were previously isolated from deep-sea vents. All strains carry virulence and antibiotic resistance genes related to those found in human pathogenic Vibrio species which may play a wider ecological role other than host infection in these environments. In addition, functional genomic analysis identified genes potentially related to deep-sea survival and stress response, alongside candidate genes encoding for novel antimicrobial agents. Ultimately, the pan-genome we generated represents a valuable resource for future studies investigating the taxonomy, evolution, and ecology of Vibrio species.
摘要：
这项研究深入研究了从太平洋深海热液喷口收集的10个弧菌菌株的基因组特征，提供对其进化史和生态适应的见解。通过涉及141种弧菌的测序和全基因组分析，我们发现深海菌株表现出具有独特基因分布的较大基因组，建议适应通风口环境。所研究的分离株的系统基因组重建揭示了2个主要进化枝的存在：第一个是单系，完全由溶藻弧菌组成，而第二个形成了一个单系进化枝，其中既包括反流弧菌，也包括空竹弧菌，以前与深海喷口隔离。所有菌株都携带与在人类致病性弧菌物种中发现的那些相关的毒力和抗生素抗性基因,其在这些环境中除了宿主感染之外还可以发挥更广泛的生态作用。此外,功能基因组分析确定了与深海生存和应激反应潜在相关的基因，以及编码新型抗微生物剂的候选基因。最终,我们生成的泛基因组是未来研究分类学的宝贵资源，进化,和弧菌物种的生态学。
