PDF(Pubmed)
Abstract：
Introduction: This study explores the therapeutic potential of silver nanoparticles (Ag NPs) synthesized using a Helianthemum lippii extract in mitigating cadmium-induced hepatotoxicity in Wistar rats. Given the increasing environmental and health concerns associated with cadmium exposure, novel and eco-friendly therapeutic strategies are essential. Methods: Ag NPs were characterized using X-ray diffraction, UV-Vis spectrometry, and energy-dispersive X-ray spectroscopy with scanning electron microscopy, confirming their formation with a cubic crystal structure and particle sizes ranging from 4.81 to 12.84 nm. A sub-acute toxicity study of Ag NPs (2 mg/kg and 10 mg/kg) was conducted, showing no significant difference compared to untreated control rats (n = 3 animals/group). Subsequently, adult Wistar rats (n = 5/group) were divided into a control group and three experimental groups: Ag NPs alone, exposure to 50 mg/kg CdCl2 in drinking water for 35 days, and CdCl2 exposure followed by 0.1 mg/kg/day Ag NPs intraperitoneally for 15 days. Results: In the CdCl2-exposed group, there was a significant decrease in body weight and increases in alanine and aspartate transaminase levels (p < 0.05 vs. control), indicating hepatotoxicity. Additionally, antioxidant defenses were decreased, and malondialdehyde levels were elevated. Liver histology revealed portal fibrosis, inflammation, necrosis, sinusoid and hepatic vein dilation, and cytoplasmic vacuolations. Treatment with Ag NPs post-CdCl2 exposure mitigated several adverse effects on liver function and architecture and improved body weight. Discussion: This study demonstrates the efficacy of Ag NPs synthesized via a green method in reducing cadmium-induced liver damage. These findings support the potential of Ag NPs in therapeutic applications and highlight the importance of sustainable and eco-friendly nanoparticle synthesis methods. By addressing both toxicity concerns and therapeutic efficacy, this research aligns with the growing emphasis on environmentally conscious practices in scientific research and healthcare.
摘要：
简介：本研究探讨了使用Helianthemumlipii提取物合成的银纳米颗粒（AgNPs）在减轻镉诱导的Wistar大鼠肝毒性中的治疗潜力。鉴于与镉接触相关的环境和健康问题日益增加，新的和生态友好的治疗策略是必不可少的。方法：使用X射线衍射对AgNPs进行表征，紫外-可见光谱法,和能量色散X射线光谱与扫描电子显微镜，确认其形成具有立方晶体结构和4.81至12.84nm的粒径。进行了AgNP（2mg/kg和10mg/kg）的亚急性毒性研究，显示与未处理的对照大鼠(n=3只动物/组)相比没有显著差异。随后，成年Wistar大鼠（n=5/组）分为对照组和三个实验组：单独使用AgNP，在饮用水中暴露于50mg/kgCdCl235天，和CdCl2暴露，然后腹膜内注射0.1mg/kg/天的AgNP，持续15天。结果：在CdCl2暴露组中，体重显着下降，丙氨酸和天冬氨酸转氨酶水平增加（p<0.05vs.control),表明肝毒性。此外,抗氧化防御能力下降，丙二醛水平升高。肝组织学显示门静脉纤维化，炎症,坏死,窦和肝静脉扩张，和细胞质空泡。CdCl2暴露后用AgNP治疗减轻了对肝功能和结构的几种不利影响并改善了体重。讨论：这项研究证明了通过绿色方法合成的AgNP在减少镉诱导的肝损伤中的功效。这些发现支持AgNP在治疗应用中的潜力，并强调了可持续和生态友好的纳米粒子合成方法的重要性。通过解决毒性问题和治疗效果，这项研究与科学研究和医疗保健中对环保意识实践的日益重视相一致。
