Abstract：
OBJECTIVE: To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.
METHODS: Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.
RESULTS: Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.
CONCLUSIONS: There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.
METHODS: Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.
RESULTS: Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.
CONCLUSIONS: There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.
摘要：
目的：研究氟中毒小鼠髁突软骨的损伤及LC3和p62的表达。
方法：将30只4周龄雄性C57BL/6小鼠随机分为对照组和实验组，每组15只。对照组给予常规饮水,实验组给予氟浓度75mg/L的饮水,连续8周。通过改良的藏红碱O-fast绿色FCF软骨染色试剂盒观察髁突软骨的结构。免疫组化法检测MMP-13、Ⅱ型胶原、LC3和p62的表达。采用SPSS22.0软件包对免疫组化半定量结果进行双向方差分析。
结果：与对照组相比，实验组的纤维软骨层变薄，软骨细胞较小，染色变得更深。免疫组化结果显示,实验组MMP-13和LC3的表达升高,Ⅱ型胶原和p62的表达降低。
结论：饮用水含有75mg/L氟化物，小鼠髁突软骨变性和自噬。
方法：将30只4周龄雄性C57BL/6小鼠随机分为对照组和实验组，每组15只。对照组给予常规饮水,实验组给予氟浓度75mg/L的饮水,连续8周。通过改良的藏红碱O-fast绿色FCF软骨染色试剂盒观察髁突软骨的结构。免疫组化法检测MMP-13、Ⅱ型胶原、LC3和p62的表达。采用SPSS22.0软件包对免疫组化半定量结果进行双向方差分析。
结果：与对照组相比，实验组的纤维软骨层变薄，软骨细胞较小，染色变得更深。免疫组化结果显示,实验组MMP-13和LC3的表达升高,Ⅱ型胶原和p62的表达降低。
结论：饮用水含有75mg/L氟化物，小鼠髁突软骨变性和自噬。
