{Reference Type}: English Abstract
{Title}: [Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse].
{Author}: Yu Y;Weng QQ;Luo YY;Yao SR;Yi FY;Zhang Y;
{Journal}: Shanghai Kou Qiang Yi Xue
{Volume}: 33
{Issue}: 2
{Year}: 2024 Apr
暂无{Abstract}: <B>OBJECTIVE: </B>To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.<BR><B>METHODS: </B>Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.<BR><B>RESULTS: </B>Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.<BR><B>CONCLUSIONS: </B>There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.