Abstract：
This study aimed to investigate the effects of the n-hexane fraction of the ethanolic seed extract of PG (NFESEPG) on hypertension induced by Nω-nitro-L-arginine methyl ester (L-NAME) in rats. Specifically, the study examined the impact of NFESEPG on blood pressure, oxidative stress markers, NO concentration, angiotensin-converting enzyme (ACE) and arginase activities, and cardiac biomarkers in hypertensive rats. The study involved collecting, identifying, and processing the PG plant to obtain the ethanolic seed extract. The extract was then partitioned with solvents to isolate the n-hexane fraction. Hypertension was induced in rats by oral administration of L-NAME for 10 days, while concurrent treatment with NFESEPG at two doses (200 and 400 mg/kg/day) was administered orally. Blood pressure was measured using a noninvasive tail-cuff method, and various biochemical parameters were assessed. Treatment with both doses of NFESEPG significantly reduced systolic and diastolic blood pressure in L-NAME-induced hypertensive rats. Additionally, NFESEPG administration increased NO concentration and decreased ACE and arginase activities, malondialdehyde (MDA) levels, and cardiac biomarkers in hypertensive rats. The findings indicate that NFESEPG effectively lowered blood pressure in hypertensive rats induced by L-NAME, potentially through mechanisms involving the modulation of oxidative stress, NO bioavailability, and cardiac biomarkers. These results suggest the therapeutic potential of NFESEPG in managing hypertension and related cardiovascular complications.
摘要：
本研究旨在探讨PG乙醇种子提取物(NFESEPG)的正己烷部分对Nω-硝基-L-精氨酸甲酯(L-NAME)诱导的大鼠高血压的影响。具体来说,这项研究检查了NFESEPG对血压的影响，氧化应激标志物，NO浓度,血管紧张素转换酶（ACE）和精氨酸酶活性，和高血压大鼠的心脏生物标志物。这项研究涉及收集，识别,并处理PG植物以获得乙醇种子提取物。然后用溶剂分配提取物以分离正己烷级分。通过口服L-NAME10天诱导大鼠高血压，同时口服两种剂量（200和400mg/kg/天）的NFESEPG治疗。血压是使用无创的尾套法测量的，和各种生化参数进行了评估。两种剂量的NFESEPG治疗均显着降低了L-NAME诱导的高血压大鼠的收缩压和舒张压。此外,NFESEPG给药增加NO浓度，降低ACE和精氨酸酶活性，丙二醛（MDA）水平，和高血压大鼠的心脏生物标志物。说明NFESEPG能有效降低L-NAME诱导的高血压大鼠的血压,可能通过涉及氧化应激调节的机制，NO生物利用度，和心脏生物标志物。这些结果表明NFESEPG在管理高血压和相关心血管并发症方面的治疗潜力。
