Abstract：
The cotton leaf hopper is a major pest in cotton, causing a hopper burn in leaves. In this study, a comparative proteomic analysis of NDLH2010 (Resistant) and LRA5166 (Susceptible), infected with leaf hopper, was employed using a nano LC-MS/MS approach. A total of 1402 proteins varied significantly between leaf hopper-infected and control plants. The resistant and susceptible genotypes had differentially expressed proteins (DEPs) of 743 and 659, respectively. Functional annotation of DEPs revealed that the DEPs were primarily associated with stress response, hormone synthesis, photosynthesis, cell wall, and secondary metabolites. Notably, DEPs such as polyphenol oxidase, carboxypeptidase, heat shock proteins, protein BTR1-like isoform X2, chaperone protein ClpB1, and β glucosidase factors associated with environmental stress response were also detected. Quantitative real-time PCR (qRT-PCR) analysis confirmed a positive correlation between protein abundances and transcripts for all genes. Collectively, this study provides the molecular mechanisms associated with cotton defense responses against leaf hopper. SIGNIFICANCE STATEMENT: Cotton, a natural fiber, assumes a pivotal role as a raw material for textile industries, thereby bearing significant importance in the global economy. The cotton production sector is considerably affected by both biotic and abiotic stresses. The cotton leaf hopper (Amrasca biguttula biguttula (Ishida)) stands as a polyphagous insect, emerging as a dominant sap-feeding pest of the cotton crop. The continuous onslaught of sap-feeding insects on cotton plants has a detrimental impact, with leaf hoppers potentially causing yield reductions of up to 50%. Therefore, comprehending the molecular interplay between cotton and leaf hopper, elucidated at the proteome level, holds promise for more effective pest management strategies. This approach holds the potential to offer insights that contribute to the development of leaf hopper-resistant cotton varieties.
摘要：
棉花叶斗是棉花的主要害虫,在树叶中造成料斗燃烧。在这项研究中,NDLH2010（抗性）和LRA5166（易感）的比较蛋白质组学分析，感染了叶斗，使用纳米LC-MS/MS方法。总共1402种蛋白质在受叶漏斗感染的植物和对照植物之间存在显着差异。抗性和易感基因型的差异表达蛋白（DEP）分别为743和659。DEP的功能注释表明，DEP主要与应激反应有关，激素合成，光合作用,细胞壁,和次生代谢产物。值得注意的是,DEP如多酚氧化酶，羧肽酶,热休克蛋白，还检测到与环境应激反应相关的蛋白BTR1样亚型X2，伴侣蛋白ClpB1和β葡萄糖苷酶因子。定量实时PCR（qRT-PCR）分析证实了所有基因的蛋白质丰度和转录本之间的正相关。总的来说，本研究提供了与棉花防御叶斗反应相关的分子机制。重要声明：棉花，天然纤维，作为纺织工业的原材料，从而在全球经济中具有重要意义。棉花生产部门受到生物和非生物胁迫的极大影响。棉叶漏斗（Amrascabiguttulabiguttula（石田））是多食昆虫，正在成为棉花作物的主要树液饲喂害虫。以树液为食的昆虫对棉株的持续猛攻产生不利影响,与叶料斗可能导致产量下降高达50％。因此,理解棉花和叶料斗之间的分子相互作用，在蛋白质组水平上阐明，有望制定更有效的虫害管理策略。这种方法具有提供见解的潜力，有助于开发抗叶斗棉花品种。
