Abstract：
Three-dimensional (3D) network provide a promising platform for construction of high sensitive electrochemical immunosensor due to the benefits of high specific surface area and electron mobility. Herein, a sensitive label-free electrochemical immunosensor based on Au nanoparticles modified Ni-B nanosheets/graphene matrix was constructed to detect diethylstilbestrol (DES). The 3D network not only could increase the electron transport rate and surface area, but also could provide confinement area, which is conducive to increases the collision frequency with the active site. Moreover, Au NPs also have good biocompatibility, which is beneficial for ligating antibodies. Benefiting from the 3D network structure and Au collective effect, the electrochemical immunosensor possess sterling detection ability with wide linear response range (0.00038-150 ng/mL) and low detection limit (31.62 fg/mL). Moreover, the constructed immunosensor can also be extend to detect DES in Tap-water and river water. This work may provide a novel material model for the construction of high sensitive immunosensor.
摘要：
由于具有高比表面积和电子迁移率的优点，三维（3D）网络为构建高灵敏度的电化学免疫传感器提供了有前途的平台。在这里，构建了基于Au纳米颗粒修饰的Ni-B纳米片/石墨烯基质的灵敏的无标记电化学免疫传感器来检测己烯雌酚（DES）。3D网络不仅可以增加电子传输速率和表面积，但也可以提供禁闭区，这有利于增加与活动部位的碰撞频率。此外，AuNPs还具有良好的生物相容性，这有利于连接抗体。受益于3D网络结构和Au集体效应，电化学免疫传感器具有较宽的线性响应范围（0.00038-150ng/mL）和较低的检测限（31.62fg/mL），具有良好的检测能力。此外，构建的免疫传感器还可以扩展到检测自来水和河水中的DES。这项工作可能为构建高灵敏度的免疫传感器提供新的材料模型。
