RESULTS: The R population evolved 4.5-fold resistance to imazamox. Acetolactate synthase (ALS) gene sequencing and ALS activity assays excluded the effect of target-site resistance in this population. P450 inhibitor malathion pretreatment significantly reversed resistance to imazamox. RNA sequencing showed that a P450 gene CYP81A104 was expressed higher in R versus susceptible (S) plants. Arabidopsis overexpressing CYP81A104 showed resistance to ALS inhibitors (imazamox, tribenuron-methyl, penoxsulam and flucarbazone-sodium), PSII inhibitor (bentazone), hydroxyphenyl pyruvate dioxygenase inhibitor (mesotrione) and auxin mimics (MCPA), which was generally consistent with the results presented in the R population.
CONCLUSIONS: This study confirmed that the CYP81A104 gene endowed resistance to multiherbicides with different modes-of-action. Our findings provide an insight into the molecular characteristics of resistance and contribute to formulating an appropriate strategy for weed management in HR crops. © 2024 Society of Chemical Industry.
结果:R种群对伊玛扎莫司的抗性提高了4.5倍。乙酰乳酸合酶(ALS)基因测序和ALS活性测定排除了该群体中靶位点抗性的影响。P450抑制剂马拉硫磷预处理显着逆转了对伊玛扎莫司的抗性。RNA测序显示,P450基因CYP81A104在R与易感(S)植物中的表达更高。过表达CYP81A104的拟南芥对ALS抑制剂(imazamox,三苯磺隆甲基,五氧磺胺和氟卡巴酮钠),PSII抑制剂(bentazone),羟苯基丙酮酸双加氧酶抑制剂(甲基磺草酮)和生长素模拟物(MCPA),这与R群体中的结果基本一致。
结论:这项研究证实,CYP81A104基因赋予了对具有不同作用方式的多种除草剂的抗性。我们的发现为抗性的分子特征提供了见解,并有助于制定适当的HR作物杂草管理策略。©2024化学工业学会。