{Reference Type}: Journal Article
{Title}: Cytochrome P450 CYP81A104 in Eleusine indica confers resistance to multiherbicide with different modes of action.
{Author}: Yao S;Yin H;Li Y;Yang Q;Yuan S;Deng W;
{Journal}: Pest Manag Sci
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jul 14
{Factor}: 4.462
{DOI}: 10.1002/ps.8310
{Abstract}: <B>BACKGROUND: </B>Developing herbicide-resistant (HR) crop cultivars is an efficient way to control weeds and minimize crop yield losses. However, widespread and long-term herbicide application has led to the evolution of resistant weeds. Here, we established a resistant (R) E. indica population, collected from imidazolinone-resistant rice cultivar fields.<BR><B>RESULTS: </B>The R population evolved 4.5-fold resistance to imazamox. Acetolactate synthase (ALS) gene sequencing and ALS activity assays excluded the effect of target-site resistance in this population. P450 inhibitor malathion pretreatment significantly reversed resistance to imazamox. RNA sequencing showed that a P450 gene CYP81A104 was expressed higher in R versus susceptible (S) plants. Arabidopsis overexpressing CYP81A104 showed resistance to ALS inhibitors (imazamox, tribenuron-methyl, penoxsulam and flucarbazone-sodium), PSII inhibitor (bentazone), hydroxyphenyl pyruvate dioxygenase inhibitor (mesotrione) and auxin mimics (MCPA), which was generally consistent with the results presented in the R population.<BR><B>CONCLUSIONS: </B>This study confirmed that the CYP81A104 gene endowed resistance to multiherbicides with different modes-of-action. Our findings provide an insight into the molecular characteristics of resistance and contribute to formulating an appropriate strategy for weed management in HR crops. © 2024 Society of Chemical Industry.