PDF(Pubmed)
Abstract：
Leaf scald, caused by Xanthomonas albilineans, is a severe disease affecting sugarcane worldwide. One of the most practical ways to control it is by developing resistant sugarcane cultivars. It is essential to identify genes associated with the response to leaf scald. A panel of 170 sugarcane genotypes was evaluated for resistance to leaf scald in field conditions for 2 years, followed by a 1-year greenhouse experiment. The phenotypic evaluation data showed a wide continuous distribution, with heritability values ranging from 0.58 to 0.84. Thirteen single nucleotide polymorphisms (SNPs) were identified, significantly associated with leaf scald resistance. Among these, eight were stable across multiple environments and association models. The candidate genes identified and validated based on RNA-seq and qRT-PCR included two genes that encode NB-ARC leucine-rich repeat (LRR)-containing domain disease-resistance protein. These findings provide a basis for developing marker-assisted selection strategies in sugarcane breeding programs.
摘要：
叶烫伤,由黄单胞菌引起的，是一种影响全球甘蔗的严重疾病。控制它的最实用方法之一是开发抗性甘蔗品种。确定与叶片烫伤反应相关的基因至关重要。对170个甘蔗基因型的小组在田间条件下对叶片烫伤的抗性进行了2年的评估，接下来是为期1年的温室实验。表型评估数据显示广泛的连续分布，遗传力值范围为0.58至0.84。鉴定出13种单核苷酸多态性(SNPs),与叶片抗烫伤性显着相关。其中,8个在多个环境和关联模型中保持稳定.基于RNA-seq和qRT-PCR鉴定和验证的候选基因包括两个编码NB-ARC富含亮氨酸重复(LRR)的结构域疾病抗性蛋白的基因。这些发现为甘蔗育种计划中开发标记辅助选择策略提供了基础。
