Abstract:
Kmeria septentrionalis is a critically endangered tree endemic to Guangxi, China, and is listed on the International Union for Conservation of Nature\'s Red List. The lack of genetic information and high-quality genome data has hindered conservation efforts and studies on this species. In this study, we present a chromosome-level genome assembly of K. septentrionalis. The genome was initially assembled to be 2.57 Gb, with a contig N50 of 11.93 Mb. Hi-C guided genome assembly allowed us to anchor 98.83% of the total length of the initial contigs onto 19 pseudochromosomes, resulting in a scaffold N50 of 135.08 Mb. The final chromosome-level genome, spaning 2.54 Gb, achieved a BUSCO completeness of 98.9% and contained 1.67 Gb repetitive elements and 35,927 coding genes. This high-quality genome assembly provides a valuable resource for understanding the genetic basis of conservation-related traits and biological properties of this endangered tree species. Furthermore, it lays a critical foundation for evolutionary studies within the Magnoliaceae family.
摘要:
Kmeriaseptentrionalis是广西特有的濒危树种,中国,并被列入国际自然保护联盟的红色名录。缺乏遗传信息和高质量的基因组数据阻碍了对该物种的保护工作和研究。在这项研究中,我们提出了K.septentrionalis的染色体水平基因组组装。基因组最初组装为2.57Gb,重叠群N50为11.93Mb。Hi-C指导的基因组组装使我们能够将初始重叠群总长度的98.83%锚定到19个假染色体上,导致支架N50为135.08Mb。最终的染色体水平基因组,跨越2.54Gb,BUSCO的完整性达到98.9%,包含1.67Gb重复元件和35,927个编码基因。这种高质量的基因组组装为了解这种濒危树种的保护相关性状和生物学特性的遗传基础提供了宝贵的资源。此外,它为木兰科的进化研究奠定了重要的基础。