{Reference Type}: Dataset {Title}: A high-quality chromosome-level genome assembly of the endangered tree Kmeria septentrionalis. {Author}: Shi CY;Qin GL;Qin YC;Lu LY;Guan DL;Gao LX; {Journal}: Sci Data {Volume}: 11 {Issue}: 1 {Year}: 2024 Jul 13 {Factor}: 8.501 {DOI}: 10.1038/s41597-024-03617-1 {Abstract}: Kmeria septentrionalis is a critically endangered tree endemic to Guangxi, China, and is listed on the International Union for Conservation of Nature's Red List. The lack of genetic information and high-quality genome data has hindered conservation efforts and studies on this species. In this study, we present a chromosome-level genome assembly of K. septentrionalis. The genome was initially assembled to be 2.57 Gb, with a contig N50 of 11.93 Mb. Hi-C guided genome assembly allowed us to anchor 98.83% of the total length of the initial contigs onto 19 pseudochromosomes, resulting in a scaffold N50 of 135.08 Mb. The final chromosome-level genome, spaning 2.54 Gb, achieved a BUSCO completeness of 98.9% and contained 1.67 Gb repetitive elements and 35,927 coding genes. This high-quality genome assembly provides a valuable resource for understanding the genetic basis of conservation-related traits and biological properties of this endangered tree species. Furthermore, it lays a critical foundation for evolutionary studies within the Magnoliaceae family.