Abstract：
BACKGROUND: Angelica sinensis (AS) and Chuanxiong rhizoma (CR) are frequently prescribed in clinical settings for their ability to enrich blood, regulate menstrual cycles, and alleviate pain. Despite their widespread use, there is a relative dearth of studies exploring their anti-inflammatory properties.
OBJECTIVE: To evaluate the antioxidant and anti-inflammatory effects of Angelica sinensis-Chuanxiong rhizoma (ASCR) extracts and investigate its anti-inflammatory mechanisms.
METHODS: AS and CR were combined in six ratios and extracted using five solvents. The quality of the resulting ASCR extracts was assessed by determining the content of ferulic acid (FA) using HPLC. The antioxidant effects of the ASCR extracts were evaluated in vitro using the DPPH and ABTS assays, as well as in HUVECs exposed to H2O2-induced oxidative damage. Additionally, the anti-inflammatory effects of the extracts were investigated in vivo through the assays of ear edema in mice and paw edema in rats. Biochemical markers including NO, MDA, and SOD in paw tissues, as well as PGE2, TNF-α, and COX-2 in rat serum, were measured to further elucidate the anti-inflammatory mechanisms of ASCR extracts.
RESULTS: The WA-2-1 was obtained by combining AS and CR in a 2:1 ratio through first water then ethanol extraction, and showed favorable antioxidant and anti-inflammatory activities. The extract demonstrated effective scavenging abilities against DPPH• and ABTS+• radicals while also protecting against H2O2-induced oxidative damage. Furthermore, in vivo studies revealed that WA-2-1 had significant inhibitory effects on ear and paw edema as well as the ability to decrease NO and MDA levels, enhance SOD activity, and downregulate the expression of COX-2, PGE2, and TNF-α.
CONCLUSIONS: The combination of AS and CR exhibits favorable anti-inflammatory effects, attributed to its dual actions of mitigating oxidative stress and suppressing the production of inflammatory mediators in serum or tissues during the inflammatory process.
OBJECTIVE: To evaluate the antioxidant and anti-inflammatory effects of Angelica sinensis-Chuanxiong rhizoma (ASCR) extracts and investigate its anti-inflammatory mechanisms.
METHODS: AS and CR were combined in six ratios and extracted using five solvents. The quality of the resulting ASCR extracts was assessed by determining the content of ferulic acid (FA) using HPLC. The antioxidant effects of the ASCR extracts were evaluated in vitro using the DPPH and ABTS assays, as well as in HUVECs exposed to H2O2-induced oxidative damage. Additionally, the anti-inflammatory effects of the extracts were investigated in vivo through the assays of ear edema in mice and paw edema in rats. Biochemical markers including NO, MDA, and SOD in paw tissues, as well as PGE2, TNF-α, and COX-2 in rat serum, were measured to further elucidate the anti-inflammatory mechanisms of ASCR extracts.
RESULTS: The WA-2-1 was obtained by combining AS and CR in a 2:1 ratio through first water then ethanol extraction, and showed favorable antioxidant and anti-inflammatory activities. The extract demonstrated effective scavenging abilities against DPPH• and ABTS+• radicals while also protecting against H2O2-induced oxidative damage. Furthermore, in vivo studies revealed that WA-2-1 had significant inhibitory effects on ear and paw edema as well as the ability to decrease NO and MDA levels, enhance SOD activity, and downregulate the expression of COX-2, PGE2, and TNF-α.
CONCLUSIONS: The combination of AS and CR exhibits favorable anti-inflammatory effects, attributed to its dual actions of mitigating oxidative stress and suppressing the production of inflammatory mediators in serum or tissues during the inflammatory process.
摘要：
背景：当归（AS）和川芎（CR）在临床环境中经常被规定为具有丰富血液的能力，调节月经周期，减轻疼痛。尽管它们广泛使用，对其抗炎特性的研究相对缺乏。
目的：评价当归川芎提取物的抗氧化和抗炎作用，并探讨其抗炎作用机制。
方法：AS和CR以六种比例混合，并使用五种溶剂提取。通过使用HPLC测定阿魏酸(FA)的含量来评估所得ASCR提取物的质量。使用DPPH和ABTS测定体外评估ASCR提取物的抗氧化作用，以及暴露于H2O2诱导的氧化损伤的HUVECs。此外,通过小鼠耳水肿和大鼠爪水肿的测定，在体内研究了提取物的抗炎作用。生化标志物包括NO,MDA,和SOD在爪组织中，以及PGE2，TNF-α，和大鼠血清中的COX-2，进行测量以进一步阐明ASCR提取物的抗炎机制。
结果：WA-2-1是通过先用水再进行乙醇提取，以2：1的比例组合AS和CR而获得的，并表现出良好的抗氧化和抗炎活性。提取物表现出对DPPH·和ABTS·自由基的有效清除能力，同时还可以抵抗H2O2诱导的氧化损伤。此外，体内研究表明，WA-2-1对耳和爪水肿有显著的抑制作用，以及降低NO和MDA水平的能力，增强SOD活性，下调COX-2、PGE2和TNF-α的表达。
结论：AS和CR的组合表现出良好的抗炎作用，归因于其减轻氧化应激和抑制炎症过程中血清或组织中炎症介质的产生的双重作用。
目的：评价当归川芎提取物的抗氧化和抗炎作用，并探讨其抗炎作用机制。
方法：AS和CR以六种比例混合，并使用五种溶剂提取。通过使用HPLC测定阿魏酸(FA)的含量来评估所得ASCR提取物的质量。使用DPPH和ABTS测定体外评估ASCR提取物的抗氧化作用，以及暴露于H2O2诱导的氧化损伤的HUVECs。此外,通过小鼠耳水肿和大鼠爪水肿的测定，在体内研究了提取物的抗炎作用。生化标志物包括NO,MDA,和SOD在爪组织中，以及PGE2，TNF-α，和大鼠血清中的COX-2，进行测量以进一步阐明ASCR提取物的抗炎机制。
结果：WA-2-1是通过先用水再进行乙醇提取，以2：1的比例组合AS和CR而获得的，并表现出良好的抗氧化和抗炎活性。提取物表现出对DPPH·和ABTS·自由基的有效清除能力，同时还可以抵抗H2O2诱导的氧化损伤。此外，体内研究表明，WA-2-1对耳和爪水肿有显著的抑制作用，以及降低NO和MDA水平的能力，增强SOD活性，下调COX-2、PGE2和TNF-α的表达。
结论：AS和CR的组合表现出良好的抗炎作用，归因于其减轻氧化应激和抑制炎症过程中血清或组织中炎症介质的产生的双重作用。
