Abstract：
Programmed cell death (PCD) is a controlled form of cell death and it plays an essential role in maintaining homeostasis. Golgi apparatus works as the hotspot during the early event of PCD and Golgi polarity, a vital microenvironment factor, can be regarded as an indicator of physiological status. Combined Golgi-targeted group phenylsulfonamide as electron acceptor group and triphenylamine as electron donor group, a novel Golgi-targeted fluorescent probe GTO had been developed. GTO showed good sensitivity and selectivity to polarity and its remarkable photostability makes it potentially useful for long-term cellular monitoring. In practice, GTO demonstrated good cell permeability and Golgi targeting capabilities. According to our results, GTO was applied to reveal the polarity increase during the early event of PCD and the encouraging results illustrated that GTO was an imaging tool for monitoring polarity in Golgi apparatus and the exploration in early diagnosis and drug discovery.
摘要：
程序性细胞死亡（PCD）是细胞死亡的受控形式，在维持稳态中起着至关重要的作用。高尔基体在PCD和高尔基极性的早期事件中作为热点，一个重要的微环境因素，可以被视为生理状态的指标。结合高尔基靶向基团苯基磺酰胺作为电子受体基团和三苯胺作为电子供体基团，一种新型的高尔基靶向荧光探针GTO已被开发。GTO对极性显示出良好的灵敏度和选择性，其出色的光稳定性使其可用于长期细胞监测。在实践中,GTO表现出良好的细胞通透性和高尔基体靶向能力。根据我们的结果,GTO用于揭示PCD早期事件期间的极性增加，令人鼓舞的结果表明，GTO是监测高尔基体极性以及早期诊断和药物发现探索的成像工具。
