Abstract：
Testicular torsion is an urological emergency and can lead to ischemia damage and testicular loss if not diagnosed in time. Proanthocyanidin is reported to have anti-inflammatory and antioxidant properties. The current study aimed to examine the possible effects of proanthocyanidin (P) on the testis in torsion/detorsion (T/D)-induced testicular ischemia/reperfusion (I/R) injury in rats. Forty rats were divided into four groups (n=10 for each): sham-operated (sham), I/R, I/R + P100 (100 mg/kg, 30 min before torsion), and I/R + P200 (200 mg/kg, 30 min before torsion). Testicular T/D was performed on the left testicle by 3 hours of torsion at 720° clockwise, followed by 3 hours of detorsion. In the I/R group, an increase in malondialdehyde (MDA) levels and a decrease in glutathione (GSH), vitamin C (Vit C), glutathione peroxidase (GPx), glucose-6-phosphate dehydrogenase (G6PD) values were determined compared to the sham group (p<0.001). Moreover, an increase in the expression of cleaved caspase-3 and Bcl2-associated X protein (Bax), a decrease in the expression of B-cell lymphoma 2 (Bcl-2) and proliferating cell nuclear antigen (PCNA) were detected in the I/R group (p<0.001). Histopathologically, it was determined that the Johnsen and Cosentino scores of the testicles were irregular in the I/R group (p<0.001). Proanthocyanidin treatment caused a decrease in MDA, cleaved caspase-3 and Bax levels and an increase in GSH, Vit C, GPx, G6PD, Bcl-2 and PCNA values. Additionally, Johnsen and Cosentino rearranged the scores. The present findings revealed the protective and curative effects of proanthocyanidin in organ damage due to testicular torsion/detorsion-induced ischemia/reperfusion with their antioxidative and antiapoptotic properties.
摘要：
睾丸扭转是泌尿外科急症，如果不及时诊断，可能导致缺血损伤和睾丸丢失。据报道原花青素具有抗炎和抗氧化特性。本研究旨在探讨原花青素（P）对扭转/扭转（T/D）诱导的大鼠睾丸缺血/再灌注（I/R）损伤的可能作用。将40只大鼠分为四组（每组n=10）：假手术（sham），I/R,I/R+P100（100mg/kg，扭转前30分钟),和I/R+P200(200毫克/千克，扭转前30分钟)。左睾丸以720°顺时针扭转3小时进行睾丸T/D，接下来是3个小时的扭曲。在I/R组中，丙二醛（MDA）水平的增加和谷胱甘肽（GSH）的减少，维生素C（VitC），谷胱甘肽过氧化物酶（GPx），与假手术组相比,测定葡萄糖-6-磷酸脱氢酶(G6PD)值(p<0.001)。此外，裂解的caspase-3和Bcl2相关X蛋白（Bax）的表达增加，在I/R组中检测到B细胞淋巴瘤2（Bcl-2）和增殖细胞核抗原（PCNA）的表达降低（p<0.001）。组织病理学,在I/R组中,睾丸的Johnsen和Cosentino评分不规则(p<0.001).原花青素处理引起MDA的降低，caspase-3和Bax水平和GSH的增加，维生素C,GPx,G6PD,Bcl-2和PCNA值。此外,约翰森和科森蒂诺重新安排了分数。本发现揭示了原花青素在睾丸扭转/扭曲引起的缺血/再灌注引起的器官损伤中的保护和治疗作用，具有抗氧化和抗凋亡特性。
