PDF(Pubmed)
Abstract：
The understanding of anti-tumor drug effects requires specific experimental settings which model clinical scenarios. We describe a protocol for 10-day treatment of lowly aggressive tumor cell lines with antineoplastic agents at concentrations which do not affect cell growth. We describe steps for seeding cells and treating cells with anti-tumor drugs. We then detail steps for cell sensitivity, cell proliferation, and mRNA and protein expression assays. We also detail assays to determine modifications in compound efflux. For complete details on the use and execution of this protocol, please refer to Rios Medrano et al.1.
摘要：
对抗肿瘤药物作用的理解需要对临床情景进行建模的特定实验设置。我们描述了一种使用浓度不影响细胞生长的抗肿瘤剂对低侵袭性肿瘤细胞系进行10天治疗的方案。我们描述了用抗肿瘤药物接种细胞和处理细胞的步骤。然后我们详细说明细胞敏感性的步骤，细胞增殖，以及mRNA和蛋白质表达测定。我们还详细介绍了确定化合物外排修饰的测定法。有关此协议的使用和执行的完整详细信息，请参考RiosMedrano等人。
