%0 Journal Article
%T Protocol for long-term treatment of lowly aggressive cancer cell lines with low concentrations of anti-tumor drugs.
%A Ríos Medrano MA
%A Prada J
%A Sequeiros MM
%A Bigi MM
%A Podestá EJ
%A Castillo AF
%A Maloberti PM
%A Orlando UD
%J STAR Protoc
%V 5
%N 3
%D 2024 Jul 12
%M 39002135
暂无%R 10.1016/j.xpro.2024.103210
%X The understanding of anti-tumor drug effects requires specific experimental settings which model clinical scenarios. We describe a protocol for 10-day treatment of lowly aggressive tumor cell lines with antineoplastic agents at concentrations which do not affect cell growth. We describe steps for seeding cells and treating cells with anti-tumor drugs. We then detail steps for cell sensitivity, cell proliferation, and mRNA and protein expression assays. We also detail assays to determine modifications in compound efflux. For complete details on the use and execution of this protocol, please refer to Rios Medrano et al.1.