RESULTS: Twenty eggs were randomly collected in the new Borg El Arab market. Bacterial isolation was carried out utilizing both traditional culture, biochemical, and PCR methods. Among the twenty eggs analyzed, three (15%) tested positive for Salmonella, while the remaining seventeen (85%) were confirmed as negative. Genotyping through multiplex PCR revealed the presence of two S. Enteritidis and other serovar, with the use of three specific gene sets: a random sequence for Salmonella spp., sdfI gene for S. Enteritidis, and flagellin (fliC gene) for S. Typhimurium. Out of the 20 isolates obtained from chicken meat, five (25%) were identified as S. Typhimurium, and three (15%) were classified as S. Enteritidis. All isolates sourced from chicken meat exhibited resistance to Rifampicin and Amoxicillin, with 90% displaying sensitivity to cefotaxime, gemifloxacin, and Erythromycin. Importantly, S. Blegdam, identified via serological methods, displayed resistance to all tested antibiotics. For the three isolates obtained from eggs, 66.6% showed sensitivity to cefotaxime, erythromycin, cefuraxime, and cefaclor, while displaying complete resistance (100%) to Amoxicillin, rifampicin, clarithromycin, and cefadroxil. Notably, one serovar exhibited absolute resistance to all tested drugs.
CONCLUSIONS: Stakeholders must implement strict control measures and rationalize antibiotic use in veterinary and human medicine due to the rise of antibiotic-resistant strains.
结果:在新的BorgElArab市场中随机收集了20个鸡蛋。细菌分离是利用传统的培养,生物化学,和PCR方法。在分析的二十个鸡蛋中,三人(15%)沙门氏菌检测呈阳性,其余17例(85%)被确认为阴性。通过多重PCR进行基因分型显示存在两种肠炎沙门氏菌和其他血清变型,使用三个特定的基因集:沙门氏菌属的随机序列。,肠炎沙门氏菌的sdfI基因,和鞭毛蛋白(fliC基因)为鼠伤寒沙门氏菌。在从鸡肉中获得的20种分离物中,五个(25%)被鉴定为鼠伤寒沙门氏菌,三个(15%)被归类为肠炎沙门氏菌。所有来自鸡肉的分离株都表现出对利福平和阿莫西林的抗性,90%对头孢噻肟敏感,吉西沙星,和红霉素.重要的是,S.布莱格丹,通过血清学方法鉴定,显示对所有测试抗生素的耐药性。对于从鸡蛋中获得的三个分离株,66.6%对头孢噻肟敏感,红霉素,头孢呋肟,和头孢克洛,同时显示对阿莫西林的完全抗性(100%),利福平,克拉霉素,和头孢羟氨苄.值得注意的是,一个血清型对所有测试药物表现出绝对抗性。
结论:由于抗生素耐药菌株的增加,利益相关者必须实施严格的控制措施,并在兽医和人类医学中合理使用抗生素。