Abstract：
BACKGROUND: The etiology of brain aneurysms remains poorly understood. Finnish research suggests that oral bacteria might contribute to the development and rupture of brain aneurysms. Previous studies by our team have not confirmed these findings, likely due to methodological differences. We aimed to replicate the Finnish study with a French population, using the same primers and comparing the results to internal controls.
METHODS: We used RT-qPCR to retrospectively analyze the expression of oral bacterial genes in eight patients. During surgical procedures, four tissue types were consistently sampled from each patient: the aneurysmal wall, the superficial temporal artery (STA), the middle meningeal artery (MMA), and the dura mater (DM). Results were expressed as fold differences employing the 2-∆∆Ct method, and statistical analyses were performed accordingly.
RESULTS: Our cohort included eight patients, evenly split into ruptured and unruptured groups. The sex distribution was balanced (4 females, 4 males). We observed DNA expression from oral bacteria in all sampled tissues; however, there were no significant differences between the ruptured and unruptured groups.
CONCLUSIONS: We detected oral bacterial gene expression in the aneurysmal wall, STA, MMA, and DM in a sample of French patients. Although limited by the small sample size, our results suggest a potential role for bacterial involvement in vascular invasiveness related to bacteremia. These findings do not definitively link oral bacteria to the pathogenesis of aneurysm development and rupture.
METHODS: We used RT-qPCR to retrospectively analyze the expression of oral bacterial genes in eight patients. During surgical procedures, four tissue types were consistently sampled from each patient: the aneurysmal wall, the superficial temporal artery (STA), the middle meningeal artery (MMA), and the dura mater (DM). Results were expressed as fold differences employing the 2-∆∆Ct method, and statistical analyses were performed accordingly.
RESULTS: Our cohort included eight patients, evenly split into ruptured and unruptured groups. The sex distribution was balanced (4 females, 4 males). We observed DNA expression from oral bacteria in all sampled tissues; however, there were no significant differences between the ruptured and unruptured groups.
CONCLUSIONS: We detected oral bacterial gene expression in the aneurysmal wall, STA, MMA, and DM in a sample of French patients. Although limited by the small sample size, our results suggest a potential role for bacterial involvement in vascular invasiveness related to bacteremia. These findings do not definitively link oral bacteria to the pathogenesis of aneurysm development and rupture.
摘要：
背景：脑动脉瘤的病因仍然知之甚少。芬兰的研究表明，口腔细菌可能有助于脑动脉瘤的发展和破裂。我们团队先前的研究尚未证实这些发现，可能是由于方法上的差异。我们的目标是用法国人口复制芬兰的研究，使用相同的引物，并将结果与内部对照进行比较。
方法：采用RT-qPCR对8例患者口腔细菌基因的表达进行回顾性分析。在外科手术过程中,从每位患者中一致取样四种组织类型：动脉瘤壁，颞浅动脉(STA),脑膜中动脉（MMA），和硬脑膜（DM）。结果表示为倍数差异，采用2-△△Ct方法，并相应进行统计分析。
结果：我们的队列包括8名患者，均匀分为破裂和未破裂组。性别分布均衡(4名女性，4名男性）。我们观察到所有样本组织中口腔细菌的DNA表达；然而，破裂组与未破裂组之间无显著差异.
结论：我们检测到动脉瘤壁中的口腔细菌基因表达，STA,MMA,和DM在法国患者样本中。尽管受到样本量小的限制，我们的结果提示细菌参与与菌血症相关的血管侵袭的潜在作用.这些发现并没有明确地将口腔细菌与动脉瘤发展和破裂的发病机理联系起来。
方法：采用RT-qPCR对8例患者口腔细菌基因的表达进行回顾性分析。在外科手术过程中,从每位患者中一致取样四种组织类型：动脉瘤壁，颞浅动脉(STA),脑膜中动脉（MMA），和硬脑膜（DM）。结果表示为倍数差异，采用2-△△Ct方法，并相应进行统计分析。
结果：我们的队列包括8名患者，均匀分为破裂和未破裂组。性别分布均衡(4名女性，4名男性）。我们观察到所有样本组织中口腔细菌的DNA表达；然而，破裂组与未破裂组之间无显著差异.
结论：我们检测到动脉瘤壁中的口腔细菌基因表达，STA,MMA,和DM在法国患者样本中。尽管受到样本量小的限制，我们的结果提示细菌参与与菌血症相关的血管侵袭的潜在作用.这些发现并没有明确地将口腔细菌与动脉瘤发展和破裂的发病机理联系起来。
