Abstract：
An isolate of a Gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium was originally isolated from soil when screening and bioprospecting for plant beneficial microorganisms. Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain was closely related to Lysinibacillus fusiformis NRRL NRS-350T (99.7%) and Lysinibacillus sphaericus NRRL B-23268T (99.2%). In phenotypic characterization, the novel strain was found to grow between 10 and 45 °C and tolerate up to 8% (w/v) NaCl. Furthermore, the strain grew in media with pH 5 to 10 (optimal growth at pH 7.0). The predominant cellular fatty acids were observed to be iso-C15: 0 (52.3%), anteiso-C15: 0 (14.8%), C16:1ω7C alcohol (11.2%), and C16: 0 (9.5%). The cell-wall peptidoglycan contained lysine-aspartic acid, the same as congeners. A draft genome was assembled and the DNA G+C content was determined to be 37.1% (mol content). A phylogenomic analysis on the core genome of the new strain and 5 closest type strains of Lysinibacillus revealed this strain formed a distinct monophyletic clade with the nearest neighbor being Lysinibacillus fusiformis. DNA-DNA relatedness studies using in silico DNA-DNA hybridizations (DDH) showed this species was below the species threshold of 70%. Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus pinottii sp. nov. is proposed, with type strain PB211T (= NRRL B-65672T, = CCUG 77181T).
摘要：
革兰氏阳性的分离株，严格的有氧，能动,杆状,内生孢子形成细菌最初是在筛选和生物勘探植物有益微生物时从土壤中分离出来的。16SrRNA基因序列的系统发育分析表明，该菌株与梭形芽孢杆菌NRRLNRS-350T（99.7％）和球形芽孢杆菌NRRLB-23268T（99.2％）密切相关。在表型表征中，发现新菌株在10至45°C之间生长，并耐受高达8％（w/v）的NaCl。此外，该菌株在pH为5至10的培养基中生长（在pH7.0下最佳生长）。观察到主要的细胞脂肪酸是异C15：0（52.3％），anteiso-C15：0（14.8%），C16：1ω7C醇（11.2%），C16:0(9.5%)。细胞壁肽聚糖含有赖氨酸-天冬氨酸，和同类人一样。组装基因组草图,并测定DNAG+C含量为37.1%(mol含量)。对新菌株的核心基因组和5个最接近的溶血芽孢杆菌菌株的系统基因组分析显示，该菌株形成了独特的单系进化枝，最近的邻居是梭形溶血芽孢杆菌。使用计算机DNA-DNA杂交（DDH）进行的DNA-DNA相关性研究表明，该物种低于70％的物种阈值。基于系统发育和表型分析的共识，我们得出的结论是，该菌株代表了Lysinibacillus属中的一种新物种，其名称为PinottiiLysinibacillussp.11月。被提议，与类型菌株PB211T（=NRRLB-65672T，=CCUG77181T）。
