Abstract：
BACKGROUND: The centromeres appear as primary constrictions on monocentric metaphase chromosomes; where sister chromatids are held together and assemble the proteinaceous kitechore complex at which microtubule proteins attach during nuclear divisions for pulling sister chromatids to opposite cell poles. The movement of chromosomes is usually governed by structural proteins that are either species-specific or highly conserved, such as the centromere-specific histone H3 (CENH3) and tubulin proteins, respectively.
RESULTS: We aimed to detect these proteins across eight different Glycine species by an immunofluorescence assay using specific antibodies. Furthermore, with the α-tubulin antibody we traced the dynamics of microtubules during the mitotic cell cycle in Glycine max. With two-color immunofluorescence staining, we showed that both proteins interact during nuclear division.
CONCLUSIONS: Finally, we proved that in different diploid and tetraploid Glycine species CENH3 can be detected in functional centromeres with spatial proximity of microtubule proteins.
RESULTS: We aimed to detect these proteins across eight different Glycine species by an immunofluorescence assay using specific antibodies. Furthermore, with the α-tubulin antibody we traced the dynamics of microtubules during the mitotic cell cycle in Glycine max. With two-color immunofluorescence staining, we showed that both proteins interact during nuclear division.
CONCLUSIONS: Finally, we proved that in different diploid and tetraploid Glycine species CENH3 can be detected in functional centromeres with spatial proximity of microtubule proteins.
摘要：
背景：着丝粒在单中心中期染色体上表现为初级收缩；其中姐妹染色单体被固定在一起并组装蛋白质kitechore复合物，微管蛋白在核分裂期间附着在该复合物上，以将姐妹染色单体拉到相反的细胞极。染色体的运动通常由物种特异性或高度保守的结构蛋白控制，如着丝粒特异性组蛋白H3（CENH3）和微管蛋白，分别。
结果：我们的目的是通过使用特异性抗体的免疫荧光测定法来检测八种不同甘氨酸物种的这些蛋白质。此外，使用α-微管蛋白抗体，我们追踪了大豆有丝分裂细胞周期中微管的动力学。用双色免疫荧光染色，我们发现两种蛋白质在核分裂过程中相互作用。
结论：最后，我们证明，在不同的二倍体和四倍体甘氨酸物种中，CENH3可以在功能着丝粒中检测到，微管蛋白在空间上接近。
结果：我们的目的是通过使用特异性抗体的免疫荧光测定法来检测八种不同甘氨酸物种的这些蛋白质。此外，使用α-微管蛋白抗体，我们追踪了大豆有丝分裂细胞周期中微管的动力学。用双色免疫荧光染色，我们发现两种蛋白质在核分裂过程中相互作用。
结论：最后，我们证明，在不同的二倍体和四倍体甘氨酸物种中，CENH3可以在功能着丝粒中检测到，微管蛋白在空间上接近。
