PDF(Pubmed)
Abstract：
Salinity stress is a common challenge in plant growth, impacting seed quality, germination, and general plant health. Sodium chloride (NaCl) ions disrupt membranes, causing ion leakage and reducing seed viability. Gibberellic acid (GA3) treatments have been found to promote germination and mitigate salinity stress on germination and plant growth. \'Bauer\' and \'Muir\' lettuce (Lactuca sativa) seeds were soaked in distilled water (control), 100 mM NaCl, 100 mM NaCl + 50 mg/L GA3, and 100 mM NaCl + 150 mg/L GA3 in Petri dishes and kept in a dark growth chamber at 25 °C for 24 h. After germination, seedlings were monitored using embedded cameras, capturing red, green, and blue (RGB) images from seeding to final harvest. Despite consistent germination rates, \'Bauer\' seeds treated with NaCl showed reduced germination. Surprisingly, the \'Muir\' cultivar\'s final dry weight differed across treatments, with the NaCl and high GA3 concentration combination yielding the poorest results (p < 0.05). This study highlights the efficacy of GA3 applications in improving germination rates. However, at elevated concentrations, it induced excessive hypocotyl elongation and pale seedlings, posing challenges for two-dimensional imaging. Nonetheless, a sigmoidal regression model using projected canopy size accurately predicted dry weight across growth stages and cultivars, emphasizing its reliability despite treatment variations (R2 = 0.96, RMSE = 0.11, p < 0.001).
摘要：
盐胁迫是植物生长面临的共同挑战,影响种子质量，发芽,和一般植物健康。氯化钠（NaCl）离子破坏膜，导致离子泄漏和降低种子活力。已发现赤霉素（GA3）处理可促进发芽并减轻盐度胁迫对发芽和植物生长的影响。\'Bauer\'和\'Muir\'生菜（Lactucasativa）种子浸泡在蒸馏水中（对照），100mMNaCl,100mMNaCl+50mg/LGA3和100mMNaCl+150mg/LGA3在培养皿中，并在25°C的黑暗生长室中保持24小时。发芽后，使用嵌入式摄像机监测幼苗，捕捉红色，绿色,和从播种到最终收获的蓝色（RGB）图像。尽管发芽率一致，用NaCl处理的\'Bauer\'种子显示发芽减少。令人惊讶的是,\'Muir\'品种的最终干重在不同的处理中有所不同，NaCl和高GA3浓度组合产生的效果最差(p<0.05)。这项研究强调了GA3应用在提高发芽率方面的功效。然而,在浓度升高的情况下，它诱导了过度的下胚轴伸长和苍白的幼苗，对二维成像提出了挑战。尽管如此，S形回归模型使用预测的冠层大小准确地预测了整个生长阶段和品种的干重，尽管治疗存在差异，但强调其可靠性(R2=0.96，RMSE=0.11，p<0.001)。
