关键词: antibacterial activity green synthesis nanofibers silver nanoparticles (AgNPs) size distribution toxicity

来  源:   DOI:10.3390/polym16131865   PDF(Pubmed)

Abstract:
This article focuses on the eco-friendly (green) synthesis of silver nanoparticles (AgNPs) and their incorporation into a polymer matrix. For AgNPs synthesis, Lavandula angustifolia (lavender) leaf extract was used as a reducing and stabilizing agent, and as a silver precursor, AgNO3 solution with different concentrations of silver (50, 100, 250, and 500 mg/L) was used. Prepared AgNPs colloids were characterized using UV-vis spectrophotometry, transmission electron microscopy (TEM), and X-ray diffraction (XRD). The spherical morphology of AgNPs with an average size of 20 nm was confirmed across all samples. Further, the antimicrobial properties of the AgNPs were evaluated using the disk diffusion method on algae (Chlorella kessleri) and the well diffusion method on bacteria (Staphylococcus chromogenes, Staphylococcus aureus, and Streptococcus uberis), along with root growth inhibition tests on white mustard (Sinapis alba). Polymer composite (PVA-AgNPs) was prepared by incorporation of AgNPs into the polymer matrix. Subsequently, non-woven textiles and thin foils were prepared. The distribution of AgNPs within the nanocomposites was observed by scanning electron microscopy (SEM). Antibacterial properties of PVA-AgNPs composites were analyzed on bacteria Streptococcus uberis. It was found that not only AgNPs showed good antimicrobial properties, but toxic properties were also transferred to the PVA-AgNPs nanocomposite.
摘要:
本文重点介绍了银纳米颗粒(AgNPs)的生态友好(绿色)合成及其在聚合物基质中的掺入。对于AgNPs合成,薰衣草(薰衣草)叶提取物被用作还原剂和稳定剂,作为银的前体,使用具有不同浓度的银(50、100、250和500mg/L)的AgNO3溶液。制备的AgNPs胶体使用紫外-可见分光光度法进行表征,透射电子显微镜(TEM),和X射线衍射(XRD)。在所有样品中确认了平均尺寸为20nm的AgNP的球形形态。Further,使用圆盘扩散法对藻类(小球藻)和细菌(葡萄球菌,金黄色葡萄球菌,和uberis链球菌),以及白芥菜(Sinapisalba)的根系生长抑制试验。聚合物复合材料(PVA-AgNPs)通过将AgNPs掺入到聚合物基质中来制备。随后,制备了非织造纺织品和薄箔。通过扫描电子显微镜(SEM)观察了纳米复合材料中AgNP的分布。分析了PVA-AgNPs复合材料对细菌链球菌的抗菌性能。发现不仅AgNP表现出良好的抗菌性能,但毒性也转移到PVA-AgNPs纳米复合材料中。
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