PDF(Pubmed)
Abstract：
The widespread prevalence of periprosthetic joint infections (PJIs) poses significant challenges in orthopedic surgeries, with pathogens such as Staphylococcus epidermidis being particularly problematic due to their capability to form biofilms on implants. This study investigates the efficacy of an innovative silver nitrate-embedded poly-L-lactide biopolymer coating designed to prevent such infections. The methods involved applying varying concentrations of silver nitrate to in vitro setups and recording the resultant bacterial growth inhibition across different serum environments, including human serum and various animal sera. Results highlighted a consistent and significant inhibition of S. epidermidis growth at all tested concentrations in each type of serum without adverse interactions with serum proteins, which commonly compromise antimicrobial efficacy. This study concludes that the silver nitrate-embedded biopolymer coating exhibits potent antibacterial properties and has potential for use in clinical settings to reduce the incidence of PJIs. Furthermore, the findings underscore the importance of considering serum interactions in the design and testing of antimicrobial implants to ensure their effectiveness in actual use scenarios. These promising results pave the way for further research to validate and refine this technology for clinical application, focusing on optimizing silver ion release and assessing biocompatibility in vivo.
摘要：
假体周围感染（PJIs）的广泛流行在骨科手术中提出了重大挑战，例如表皮葡萄球菌的病原体由于它们在植入物上形成生物膜的能力而特别成问题。这项研究调查了旨在防止此类感染的创新硝酸银嵌入的聚L-丙交酯生物聚合物涂层的功效。该方法涉及将不同浓度的硝酸银应用于体外设置，并记录在不同血清环境中产生的细菌生长抑制，包括人血清和各种动物血清。结果表明，在每种类型的血清中，在所有测试浓度下，表皮葡萄球菌的生长均具有一致且显着的抑制作用，而与血清蛋白没有不利的相互作用。这通常会损害抗菌功效。这项研究得出的结论是，硝酸银嵌入的生物聚合物涂层表现出有效的抗菌性能，并有可能在临床环境中使用，以减少PJI的发生率。此外，研究结果强调了在抗菌植入物的设计和测试中考虑血清相互作用的重要性,以确保其在实际使用场景中的有效性.这些有希望的结果为进一步的研究铺平了道路，以验证和完善该技术的临床应用，专注于优化银离子释放和评估体内生物相容性。
