PDF(Pubmed)
Abstract：
Sarcopenia refers to an age-related decrease in muscle mass and strength. The gut-muscle axis has been proposed as a promising target to alleviate muscle atrophy. The effect of KL-Biome-a postbiotic preparation comprising heat-killed Lactiplantibacillus plantarum KM-2, its metabolites, and an excipient (soybean powder)-on muscle atrophy was evaluated using dexamethasone (DEX)-induced atrophic C2C12 myoblasts and C57BL/6J mice. KL-Biome significantly downregulated the expression of genes (Atrogin-1 and MuRF1) associated with skeletal muscle degradation but increased the anabolic phosphorylation of FoxO3a, Akt, and mTOR in C2C12 cells. Oral administration of KL-Biome (900 mg/kg) for 8 weeks significantly improved muscle mass, muscle function, and serum lactate dehydrogenase levels in DEX-treated mice. KL-Biome administration increased gut microbiome diversity and reversed DEX-mediated gut microbiota alterations. Furthermore, it significantly increased the relative abundances of the genera Subdologranulum, Alistipes, and Faecalibacterium prausnitzii, which are substantially involved in short-chain fatty acid production. These findings suggest that KL-Biome exerts beneficial effects on muscle atrophy by regulating gut microbiota.
摘要：
肌肉减少是指与年龄相关的肌肉质量和力量的降低。已经提出肠-肌肉轴作为缓解肌肉萎缩的有希望的目标。KL-Biome-a后生物制剂的作用，该制剂包含热灭活的植物乳杆菌KM-2，其代谢产物，使用地塞米松（DEX）诱导的萎缩性C2C12成肌细胞和C57BL/6J小鼠评估了赋形剂（大豆粉）对肌肉萎缩的作用。KL-Biome显着下调与骨骼肌降解相关的基因（Atrogin-1和MuRF1）的表达，但增加了FoxO3a的合成代谢磷酸化，Akt,和C2C12细胞中的mTOR。口服KL-Biome（900mg/kg）8周显着改善肌肉质量，肌肉功能，和DEX治疗小鼠的血清乳酸脱氢酶水平。KL-Biome施用增加了肠道微生物组多样性并逆转了DEX介导的肠道微生物群改变。此外，它显著增加了亚球形颗粒属的相对丰度，Alistipes,和prausnitzii粪杆菌,它们基本上参与短链脂肪酸的生产。这些发现表明KL-Biome通过调节肠道微生物群对肌肉萎缩发挥有益作用。
