PDF(Pubmed)
Abstract：
Plants are often exposed to biotic or abiotic stress, which can seriously impede their growth and development. In recent years, researchers have focused especially on the study of plant responses to biotic and abiotic stress. As one of the most widely planted grapevine rootstocks, \'Beta\' has been extensively proven to be highly resistant to stress. However, further research is needed to understand the mechanisms of abiotic stress in \'Beta\' rootstocks. In this study, we isolated and cloned a novel WRKY transcription factor, VhWRKY44, from the \'Beta\' rootstock. Subcellular localization analysis revealed that VhWRKY44 was a nuclear-localized protein. Tissue-specific expression analysis indicated that VhWRKY44 had higher expression levels in grape roots and mature leaves. Further research demonstrated that the expression level of VhWRKY44 in grape roots and mature leaves was highly induced by salt and cold treatment. Compared with the control, Arabidopsis plants overexpressing VhWRKY44 showed stronger resistance to salt and cold stress. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were significantly increased, and the contents of proline, malondialdehyde (MDA) and chlorophyll were changed considerably. In addition, significantly higher levels of stress-related genes were detected in the transgenic lines. The results indicated that VhWRKY44 was an important transcription factor in \'Beta\' with excellent salt and cold tolerance, providing a new foundation for abiotic stress research.
摘要：
植物经常暴露于生物或非生物胁迫，这会严重阻碍他们的成长和发展。近年来,研究人员特别关注植物对生物和非生物胁迫的反应。作为种植最广泛的葡萄砧木之一，\'Beta\'已被广泛证明对压力具有很强的抵抗力。然而,需要进一步的研究来了解β砧木非生物胁迫的机制。在这项研究中,我们分离并克隆了一个新的WRKY转录因子，VhWRKY44，来自“贝塔”砧木。亚细胞定位分析显示VhWRKY44是核定位蛋白。组织特异性表达分析表明，VhWRKY44在葡萄根和成熟叶中具有较高的表达水平。进一步研究表明，盐和冷处理高度诱导了VhWRKY44在葡萄根和成熟叶中的表达水平。与对照相比，过表达VhWRKY44的拟南芥植株对盐和冷胁迫表现出较强的抗性。超氧化物歧化酶(SOD)活性,过氧化物酶(POD),过氧化氢酶（CAT）显著增加，和脯氨酸的含量，丙二醛（MDA）和叶绿素发生了很大变化。此外,在转基因品系中检测到明显更高水平的应激相关基因。结果表明，VhWRKY44是β中重要的转录因子，具有优异的耐盐性和耐寒性，为非生物胁迫研究提供了新的基础。
