PDF(Pubmed)
Abstract：
Selenium (Se)-rich Cyclocarya paliurus is popular for its bioactive components, and exogenous Se fortification is the most effective means of enrichment. However, the effects of exogenous Se fortification on the nutritional quality of C. paliurus are not well known. To investigate the nutrient contents and antioxidant properties of C. paliurus following Se treatment, we used a foliar spray to apply Se in two forms-chemical nano-Se (Che-SeNPs) and sodium selenite (Na2SeO3). Sampling began 10 days after spraying and was conducted every 5 days until day 30. The Se, secondary metabolite, malondialdehyde contents, antioxidant enzyme activity, Se speciation, and Se-metabolism-related gene expression patterns were analyzed in the collected samples. Exogenous Se enhancement effectively increased the Se content of leaves, reaching a maximum on days 10 and 15 of sampling, while the contents of flavonoids, triterpenes, and polyphenols increased significantly during the same period. In addition, the application of Se significantly enhanced total antioxidant activity, especially the activity of the antioxidant enzyme peroxidase. Furthermore, a positive correlation between the alleviation of lipid peroxidation and Se content was observed, while methylselenocysteine formation was an effective means of alleviating Se stress. Finally, Na2SeO3 exhibited better absorption and conversion efficiency than Che-SeNPs in C. paliurus.
摘要：
富含硒(Se)的青钱柳因其生物活性成分而备受欢迎,外源硒强化是最有效的富集手段。然而,外源硒强化对鲍鱼营养品质的影响尚不清楚。研究硒处理后黄柳的营养成分和抗氧化性能。我们使用叶面喷雾以两种形式施用Se-化学纳米Se（Che-SeNP）和亚硒酸钠（Na2SeO3）。在喷雾后10天开始取样,每5天进行一次,直到第30天。Se,次生代谢产物,丙二醛含量，抗氧化酶活性，硒形态，在收集的样品中分析了硒代谢相关基因的表达模式。外源硒的增强有效提高了叶片的硒含量,在采样的第10天和第15天达到最大值，而类黄酮的含量，三萜,多酚在同一时期显著增加。此外,硒的应用显著增强了总抗氧化活性,尤其是抗氧化酶过氧化物酶的活性。此外，观察到脂质过氧化的减轻与硒含量之间呈正相关，而甲基硒代半胱氨酸的形成是缓解硒胁迫的有效手段。最后,Na2SeO3在巴柳中表现出比Che-SeNP更好的吸收和转化效率。
