PDF(Pubmed)
Abstract：
The capsule-associated protein 10 gene (CAP10) is indispensable due to its involvement in pod formation and virulence maintenance in Cryptococcus neoformans. The function of the CAP10 gene in nematode-predatory fungi remains unreported. As a typical nematode-trapping fungus, Dactylellina haptotyla efficiently captures nematodes using adhesive knobs, which has potential applications in the biological control of plant-parasitic nematodes. In this study, we investigated the function of DHXT1 (a CAP10 homologous protein) in D. haptotyla-nematode interactions based on the disruption and overexpression of DHXT1, phenotypic analysis and metabolomic analysis. As a result, it was shown that the disruption of the DHXT1 gene causes a marked decrease in the number of adhesive knobs, and on the contrary, the overexpression of the DHXT1 gene causes a substantial increase in the number of adhesive knobs. Interestingly, the variety of metabolites increased with the disruption of the DHXT1 and decreased with the overexpression of the DHXT1 gene. The results suggest that DHXT1 effects pathogenicity through its involvement in adhesive knobs\' formation and metabolite synthesis and serves as a key virulence factor in D. haptotyla.
摘要：
胶囊相关蛋白10基因（CAP10）是必不可少的，因为它参与了新型隐球菌的荚形成和毒力维持。CAP10基因在线虫掠食性真菌中的功能仍未报道。作为一种典型的捕获线虫的真菌,使用粘合剂旋钮有效地捕获线虫，在植物寄生线虫的生物防治中具有潜在的应用前景。在这项研究中,我们基于DHXT1的破坏和过表达，表型分析和代谢组学分析，研究了DHXT1（CAP10同源蛋白）在D.hapotyla-线虫相互作用中的功能。因此,研究表明，DHXT1基因的破坏导致粘附旋钮的数量显着减少，相反,DHXT1基因的过表达导致粘附旋钮数量的大量增加。有趣的是,代谢物的种类随着DHXT1的破坏而增加，随着DHXT1基因的过表达而减少。结果表明，DHXT1通过参与粘附旋钮的形成和代谢物的合成而影响致病性，并作为D.haptotyla的关键毒力因子。
