PDF(Pubmed)
Abstract：
Postmenopausal osteoporosis, characterized by an imbalance between osteoclast-mediated bone resorption and osteoblast-driven bone formation, presents substantial health implications. In this study, we investigated the role of black goat extract (BGE), derived from a domesticated native Korean goat, estrogen-like activity, and osteoprotective effects in vitro. BGE\'s mineral and fatty acid compositions were analyzed via the ICP-AES method and gas chromatography-mass spectrometry, respectively. In vitro experiments were conducted using MCF-7 breast cancer cells, MC3T3-E1 osteoblasts, and RAW264.7 osteoclasts. BGE exhibits a favorable amount of mineral and fatty acid content. It displayed antimenopausal activity by stimulating MCF-7 cell proliferation and augmenting estrogen-related gene expression (ERα, ERβ, and pS2). Moreover, BGE positively impacted osteogenesis and mineralization in MC3T3-E1 cells through Wnt/β-catenin pathway modulation, leading to heightened expression of Runt-related transcription factor 2, osteoprotegerin, and collagen type 1. Significantly, BGE effectively suppressed osteoclastogenesis by curtailing osteoclast formation and activity in RAW264.7 cells, concurrently downregulating pivotal signaling molecules, including receptor activator of nuclear factor κ B and tumor necrosis factor receptor-associated factor 6. This study offers a shred of preliminary evidence for the prospective use of BGE as an effective postmenopausal osteoporosis treatment.
摘要：
绝经后骨质疏松症,以破骨细胞介导的骨吸收和成骨细胞驱动的骨形成之间的不平衡为特征，对健康有重大影响。在这项研究中,我们调查了黑山羊提取物（BGE）的作用，源自一只驯化的韩国本土山羊，雌激素样活性，和体外骨保护作用。通过ICP-AES方法和气相色谱-质谱法分析了BGE的矿物质和脂肪酸组成，分别。使用MCF-7乳腺癌细胞进行体外实验，MC3T3-E1成骨细胞，和RAW264.7破骨细胞。BGE表现出有利量的矿物质和脂肪酸含量。它通过刺激MCF-7细胞增殖和增加雌激素相关基因表达而表现出抗绝经期活性（ERα，ERβ,和pS2）。此外，BGE通过Wnt/β-catenin通路调节正向影响MC3T3-E1细胞成骨和矿化,导致Runt相关转录因子2，骨保护素，和胶原蛋白类型1。重要的是,BGE通过减少RAW264.7细胞中破骨细胞的形成和活性，有效抑制破骨细胞的生成，同时下调关键信号分子，包括核因子κB受体活化因子和肿瘤坏死因子受体相关因子6。这项研究为BGE作为绝经后骨质疏松症的有效治疗提供了初步证据。
