PDF(Pubmed)
Abstract：
This study assessed the biocompatibility of two types of nanogold composites: fibronectin-gold (FN-Au) and collagen-gold (Col-Au). It consisted of three main parts: surface characterization, in vitro biocompatibility assessments, and animal models. To determine the structural and functional differences between the materials used in this study, atomic force microscopy, Fourier-transform infrared spectroscopy, and ultraviolet-visible spectrophotometry were used to investigate their surface topography and functional groups. The F-actin staining, proliferation, migration, reactive oxygen species generation, platelet activation, and monocyte activation of mesenchymal stem cells (MSCs) cultured on the FN-Au and Col-Au nanocomposites were investigated to determine their biological and cellular behaviors. Additionally, animal biocompatibility experiments measured capsule formation and collagen deposition in female Sprague-Dawley rats. The results showed that MSCs responded better on the FN-Au and Col-AU nanocomposites than on the control (tissue culture polystyrene) or pure substances, attributed to their incorporation of an optimal Au concentration (12.2 ppm), which induced significant surface morphological changes, nano topography cues, and better biocompatibility. Moreover, neuronal, endothelial, bone, and adipose tissues demonstrated better differentiation ability on the FN-Au and Col-Au nanocomposites. Nanocomposites have a crucial role in tissue engineering and even vascular grafts. Finally, MSCs were demonstrated to effectively enhance the stability of the endothelial structure, indicating that they can be applied as promising alternatives to clinics in the future.
摘要：
这项研究评估了两种类型的纳米金复合材料的生物相容性：纤连蛋白-金（FN-Au）和胶原蛋白-金（Col-Au）。它由三个主要部分组成：表面表征，体外生物相容性评估，和动物模型。为了确定本研究中使用的材料之间的结构和功能差异，原子力显微镜,傅里叶变换红外光谱,和紫外可见分光光度法用于研究它们的表面形貌和官能团。F-肌动蛋白染色，扩散,迁移,活性氧的产生，血小板活化,研究了在FN-Au和Col-Au纳米复合材料上培养的间充质干细胞（MSCs）的单核细胞活化，以确定其生物学和细胞行为。此外,动物生物相容性实验测量雌性Sprague-Dawley大鼠的胶囊形成和胶原蛋白沉积。结果表明，MSCs对FN-Au和Col-AU纳米复合材料的反应优于对照（组织培养聚苯乙烯）或纯物质，归因于它们掺入了最佳Au浓度（12.2ppm），这引起了显著的表面形态变化，纳米形貌线索，和更好的生物相容性。此外，神经元，内皮，骨头,和脂肪组织在FN-Au和Col-Au纳米复合材料上表现出更好的分化能力。纳米复合材料在组织工程甚至血管移植中起着至关重要的作用。最后,MSCs被证明能有效增强内皮结构的稳定性,这表明它们将来可以作为有希望的诊所替代品。
