PDF(Pubmed)
Abstract：
Drought stress is a major meteorological threat to crop growth and yield. Barley (Hordeum vulgare L.) is a vital cereal crop with strong drought tolerance worldwide. However, the underlying growth properties and metabolomic regulatory module of drought tolerance remains less known. Here, we investigated the plant height, spike length, effective tiller, biomass, average spikelets, 1000-grain weight, number of seeds per plant, grain weight per plant, ash content, protein content, starch content, cellulose content, and metabolomic regulation mechanisms of drought stress in barley. Our results revealed that the growth properties were different between ZDM5430 and IL-12 under drought stress at different growth stages. We found that a total of 12,235 metabolites were identified in two barley genotype root samples with drought treatment. More than 50% of these metabolites showed significant differences between the ZDM5430 and IL-12 roots. The Kyoto Encyclopedia of Genes and Genomes pathway analysis identified 368 differential metabolites mainly involved in starch and sucrose metabolism, the pentose phosphate pathway, pyrimidine metabolism, phenylalanine, tyrosine, and tryptophan biosynthesis in ZDM5430 under drought stress, whereas the different metabolites of IL-12 under drought stress related to starch and sucrose metabolism, the pentose phosphate pathway, 2-oxocarboxylic acid metabolism, cutin, suberine and wax biosynthesis, carbon metabolism, fatty acid biosynthesis, and C5-branched dibasic acid metabolism. These metabolites have application in the tricarboxylic cycle, the urea cycle, the met salvage pathway, amino acid metabolism, unsaturated fatty acid biosynthesis, phenolic metabolism, and glycolysis. On the other hand, the expression patterns of 13 genes related to the abovementioned bioprocesses in different barley genotypes roots were proposed. These findings afford an overview for the understanding of barley roots\' metabolic changes in the drought defense mechanism by revealing the differently accumulated compounds.
摘要：
干旱胁迫是作物生长和产量的主要气象威胁。大麦（HordeumvulgareL.）是一种重要的谷物作物，在世界范围内具有很强的耐旱性。然而,耐旱性的潜在生长特性和代谢组学调节模块仍然鲜为人知。这里,我们调查了植物高度，穗长,有效耕作机,生物量，平均小穗，1000粒重，每株植物的种子数量，每株植物的粒重，灰分含量,蛋白质含量，淀粉含量,纤维素含量，大麦干旱胁迫的代谢组学调控机制。我们的结果表明，ZDM5430和IL-12在不同生长阶段的干旱胁迫下生长特性不同。我们发现在干旱处理的两个大麦基因型根样品中总共鉴定出12,235种代谢物。超过50％的这些代谢物在ZDM5430和IL-12根之间显示出显着差异。京都百科全书的基因和基因组途径分析确定了368个差异代谢物主要涉及淀粉和蔗糖代谢，磷酸戊糖途径，嘧啶代谢，苯丙氨酸,酪氨酸,干旱胁迫下ZDM5430中色氨酸的生物合成，而干旱胁迫下IL-12的不同代谢产物与淀粉和蔗糖代谢有关,磷酸戊糖途径，2-氧代羧酸代谢，角质,苏木和蜡的生物合成，碳代谢，脂肪酸生物合成，和C5-支链二元酸代谢。这些代谢物在三羧酸循环中有应用，尿素循环，相遇的救助途径，氨基酸代谢，不饱和脂肪酸生物合成，酚类代谢，和糖酵解。另一方面,提出了与上述生物过程相关的13个基因在不同基因型大麦根中的表达模式。这些发现通过揭示不同积累的化合物，为理解大麦根系在干旱防御机制中的代谢变化提供了概述。
