PDF(Pubmed)
Abstract：
One of the most significant diseases in the swine business, porcine reproductive and respiratory syndrome virus (PRRSV) causes respiratory problems in piglets and reproductive failure in sows. The PRRSV nucleocapsid (N) protein is essential for the virus\' assembly, replication, and immune evasion. Stages in the viral replication cycle can be impacted by interactions between the PRRSV nucleocapsid protein and the host protein components. Therefore, it is of great significance to explore the interaction between the PRRSV nucleocapsid protein and the host. Nevertheless, no information has been published on the network of interactions between the nucleocapsid protein and the host proteins in primary porcine alveolar macrophages (PAMs). In this study, 349 host proteins interacting with nucleocapsid protein were screened in the PRRSV-infected PAMs through a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics approach. Bioinformatics analysis, which included gene ontology annotation, Kyoto Encyclopedia of Genes and Genomes database enrichment, and a protein-protein interaction (PPI) network, revealed that the host proteins interacting with PRRSV-N may be involved in protein binding, DNA transcription, metabolism, and innate immune responses. This study confirmed the interaction between the nucleocapsid protein and the natural immune-related proteins. Ultimately, our findings suggest that the nucleocapsid protein plays a pivotal role in facilitating immune evasion during a PRRSV infection. This study contributes to enhancing our understanding of the role played by the nucleocapsid protein in viral pathogenesis and virus-host interaction, thereby offering novel insights for the prevention and control of PRRS as well as the development of vaccines.
摘要：
养猪业中最重要的疾病之一，猪繁殖与呼吸综合征病毒(PRRSV)导致仔猪呼吸问题和母猪繁殖障碍。PRRSV核衣壳(N)蛋白是病毒组装所必需的,复制,和免疫逃避。病毒复制周期中的阶段可受到PRRSV核衣壳蛋白与宿主蛋白组分之间的相互作用的影响。因此,探索PRRSV核衣壳蛋白与宿主的相互作用具有重要意义。然而，关于猪原代肺泡巨噬细胞(PAMs)中核衣壳蛋白与宿主蛋白之间相互作用的网络,目前尚无相关信息.在这项研究中,通过基于液相色谱-串联质谱（LC-MS/MS）的蛋白质组学方法，在PRRSV感染的PAMs中筛选了349种与核衣壳蛋白相互作用的宿主蛋白。生物信息学分析，其中包括基因本体论注释，京都基因和基因组数据库富集百科全书，和蛋白质-蛋白质相互作用（PPI）网络，表明与PRRSV-N相互作用的宿主蛋白可能参与蛋白结合，DNA转录，新陈代谢，和先天免疫反应。这项研究证实了核衣壳蛋白与天然免疫相关蛋白之间的相互作用。最终,我们的研究结果表明,核衣壳蛋白在促进PRRSV感染期间的免疫逃避中起关键作用.这项研究有助于增强我们对核衣壳蛋白在病毒发病机理和病毒与宿主相互作用中的作用的理解。从而为PRRS的预防和控制以及疫苗的开发提供了新的见解。
