PDF(Pubmed)
Abstract：
Quorum sensing (QS) allows bacteria to coordinate their activities by producing and detecting low-molecular-weight signal molecules based on population density, thereby controlling the infectivity of bacteria through various virulence factors. Quorum-sensing inhibition is a promising approach to tackle bacterial communication. Cyclodextrins (CDs) are a class of cyclic oligosaccharides that reversibly encapsulate the acyl chain of the signal molecules, thereby preventing their binding to receptors and interrupting bacterial communication. This results in the inhibition of the expression of various properties, including different virulence factors. To examine the potential quorum-quenching (QQ) ability of newly prepared cyclodextrin derivatives, we conducted short-term tests using Aliivibrio fischeri, a heterotrophic marine bacterium capable of bioluminescence controlled by quorum sensing. α- and β-cyclodextrins monosubstituted with alkylthio moieties and further derivatized with quaternary ammonium groups were used as the test agents. The effect of these cyclodextrins on the quorum-sensing system of A. fischeri was investigated by adding them to an exponential growth phase of the culture and then measuring bioluminescence intensity, population growth, and cell viability. Our results demonstrate that the tested cyclodextrins have an inhibitory effect on the quorum-sensing system of A. fischeri. The inhibitory effect varies based on the length of the alkyl chain, with alkylthio substitution enhancing it and the presence of quaternary ammonium groups decreasing it. Our findings suggest that cyclodextrins can be a promising therapeutic agent for the treatment of bacterial infections.
摘要：
群体感应（QS）允许细菌通过基于种群密度产生和检测低分子量信号分子来协调其活动，从而通过各种毒力因子控制细菌的感染性。群体感应抑制是解决细菌通讯的一种有前途的方法。环糊精（CD）是一类环状寡糖，可逆地包裹信号分子的酰基链，从而阻止它们与受体结合并中断细菌交流。这导致抑制各种性质的表达，包括不同的毒力因子。为了检查新制备的环糊精衍生物的潜在群体猝灭（QQ）能力，我们用费氏阿利弧菌进行了短期试验，一种异养海洋细菌，能够通过群体感应控制生物发光。用烷硫基部分单取代并进一步用季铵基团衍生的α-和β-环糊精用作测试试剂。通过将它们添加到培养物的指数生长期，然后测量生物发光强度，研究了这些环糊精对A.fischeri群体感应系统的影响。人口增长,和细胞活力。我们的结果表明，测试的环糊精对费氏酵母的群体感应系统具有抑制作用。抑制作用根据烷基链的长度而变化，烷硫基取代会增强它，而季铵基团的存在会减少它。我们的发现表明，环糊精可以成为治疗细菌感染的有前途的治疗剂。
