Abstract:
In this study, we present data on the effects of condensed tannins (CTs) and hydrolysable tannins (HTs), polyphenols extracted from plants, at different concentrations on zebrafish development to identify the range of concentrations with toxic effects. Zebrafish embryos were exposed to CTs and HTs at two different concentration ranges (5.0-20.0 μgL-1 and 5.0-20.0 mgL-1) for 72 h. The toxicity parameters were observed up to 72 h of treatment. The uptake of CTs and HTs by the zebrafish larvae was assessed via HPLC analysis. A qRT-PCR analysis was performed to evaluate the expressions of genes cd63, zhe1, and klf4, involved in the hatching process of zebrafish. CTs and HTs at 5.0, 10.0, and 20.0 μgL-1 were not toxic. On the contrary, at 5.0, 10.0, and 20.0 mgL-1, HTs induced a delay in hatching starting from 48 h of treatment, while CTs showed a delay in hatching mainly at 48 h. The analysis of gene expression showed a downregulation in the group exposed to HTs, confirming the hatching data. We believe that this study is important for defining the optimal doses of CTs and HTs to be employed in different application fields such as the chemical industry, the animal feed industry, and medical science.
摘要:
在这项研究中,我们提供了有关缩合单宁(CTs)和可水解单宁(HTs)作用的数据,从植物中提取的多酚,在不同浓度下对斑马鱼发育有毒性作用的浓度范围进行鉴定。斑马鱼胚胎暴露于两种不同浓度范围(5.0-20.0μgL-1和5.0-20.0mgL-1)的CT和HT72h。观察到毒性参数长达72h的处理。通过HPLC分析评估斑马鱼幼虫对CT和HT的摄取。进行qRT-PCR分析以评估参与斑马鱼孵化过程的基因cd63,zhe1和klf4的表达。5.0、10.0和20.0μgL-1的CT和HT是无毒的。相反,在5.0、10.0和20.0mgL-1时,HTs从处理48小时开始诱导孵化延迟,而CT显示孵化延迟主要在48小时。基因表达的分析显示在暴露于HTs的组中下调,确认孵化数据。我们认为,这项研究是重要的确定的最佳剂量的CT和HTs被用于不同的应用领域,如化学工业,动物饲料工业,和医学科学。