PDF(Pubmed)
Abstract：
Global public health is facing a major issue with emerging resistance to antimicrobial agents. Antimicrobial agents that are currently on the market are strong and efficient, but it has not been ruled out that these medications will eventually cause resistance to bacteria. Exploring novel bioactive compounds derived from natural sources is therefore, crucial to meet future demands. The present study evaluated the mode of action of the antimicrobial potential protease enzyme SH21. Protease SH21 exhibited antimicrobial activity, strong heat stability (up to 100 °C), and pH stability (pH 3.0 to 9.0). In terms of mode of action, we found that protease SH21 was able to disrupt the bacterial cell membrane as the results of the nucleotide leakage and cell membrane permeability assay. In addition, we also checked inner membrane permeability by PI uptake assay which suggested that protease SH21 has the ability to enter the bacterial cell membrane. Our results revealed that the antimicrobial protease SH21 might be a promising candidate for treating microbial infections.
摘要：
全球公共卫生正面临着新出现的抗微生物药物耐药性的重大问题。目前市场上的抗菌药物是强大而有效的，但并不排除这些药物最终会对细菌产生抗药性。因此，探索源自天然来源的新型生物活性化合物，对满足未来需求至关重要。本研究评估了抗微生物潜在蛋白酶SH21的作用方式。蛋白酶SH21表现出抗菌活性，热稳定性强（高达100°C），和pH稳定性(pH3.0至9.0)。就行动方式而言,我们发现，蛋白酶SH21能够破坏细菌细胞膜的核苷酸渗漏和细胞膜通透性测定的结果。此外,我们还通过PI摄取测定法检查了内膜通透性，这表明蛋白酶SH21具有进入细菌细胞膜的能力。我们的结果表明，抗微生物蛋白酶SH21可能是治疗微生物感染的有希望的候选者。
