PDF(Pubmed)
Abstract：
Plastic particles, particularly micro- and nanoparticles, are emerging pollutants due to the ever-growing amount of plastics produced across a wide variety of sectors. When plastic particles enter a biological medium, they become surrounded by a corona, giving them their biological identity and determining their interactions in the living environment and their biological effects. Here, we studied the interactions of microstructured plastics with hemoglobin (Hb). Virgin polyethylene microparticles (PEMPs) and polypropylene microparticles (PPMPs) as well as heat- or irradiation-aged microparticles (ag-PEMPs and ag-PPMPs) were used to quantify Hb adsorption. Polypropylene filters (PP-filters) were used to measure the oxygenation of adsorbed Hb. Microstructured plastics were characterized using optical microscopy, SAXS, ATR-FTIR, XPS, and Raman spectroscopy. Adsorption isotherms showed that the Hb corona thickness is larger on PPMPs than on PEMPs and Hb has a higher affinity for PPMPs than for PEMPs. Hb had a lower affinity for ag-PEMPs and ag-PPMPs, but they can be adsorbed in larger amounts. The presence of partial charges on the plastic surface and the oxidation rate of microplastics may explain these differences. Tonometry experiments using an original method, the diffuse reflection of light, showed that adsorbed Hb on PP-filters retains its cooperativity, but its affinity for O2 decreases significantly.
摘要：
塑料颗粒,特别是微米和纳米颗粒，由于各种行业生产的塑料数量不断增加，它们正在出现污染物。当塑料颗粒进入生物介质时，它们被日冕包围，赋予他们生物学身份，并确定他们在生活环境中的相互作用及其生物学效应。这里,我们研究了微结构塑料与血红蛋白（Hb）的相互作用。使用原始聚乙烯微粒（PEMP）和聚丙烯微粒（PPMP）以及热老化或辐照老化的微粒（ag-PEMP和ag-PPMP）来量化Hb吸附。聚丙烯过滤器(PP过滤器)用于测量吸附的Hb的氧合。使用光学显微镜对微结构塑料进行了表征，SAXS,ATR-FTIR,XPS,和拉曼光谱。吸附等温线表明，PPMPs上的Hb电晕厚度大于PEMPs，并且Hb对PPMPs的亲和力高于PEMPs。Hb对ag-PEMPs和ag-PPMPs的亲和力较低，但是它们可以被大量吸附。塑料表面上部分电荷的存在和微塑料的氧化速率可以解释这些差异。使用原始方法的眼压实验，光的漫反射，表明吸附在PP过滤器上的Hb保持其协同性，但其对O2的亲和力显著下降。
