PDF(Pubmed)
Abstract：
Sulfur metabolism plays a major role in plant growth and development, environmental adaptation, and material synthesis, and the sulfate transporters are the beginning of sulfur metabolism. We identified 37 potential VcSULTR genes in the blueberry genome, encoding peptides with 534 to 766 amino acids. The genes were grouped into four subfamilies in an evolutionary analysis. The 37 putative VcSULTR proteins ranged in size from 60.03 to 83.87 kDa. These proteins were predicted to be hydrophobic and mostly localize to the plasma membrane. The VcSULTR genes were distributed on 30 chromosomes; VcSULTR3;5b and VcSULTR3;5c were the only tandemly repeated genes. The VcSULTR promoters contained cis-acting elements related to the fungal symbiosis and stress responses. The transcript levels of the VcSULTRs differed among blueberry organs and changed in response to ericoid mycorrhizal fungi and sulfate treatments. A subcellular localization analysis showed that VcSULTR2;1c localized to, and functioned in, the plasma membrane and chloroplast. The virus-induced gene knock-down of VcSULTR2;1c resulted in a significantly decreased endogenous sulfate content, and an up-regulation of genes encoding key enzymes in sulfur metabolism (VcATPS2 and VcSiR1). These findings enhance our understanding of mycorrhizal-fungi-mediated sulfate transport in blueberry, and lay the foundation for further research on blueberry-mycorrhizal symbiosis.
摘要：
硫代谢在植物生长发育中起着重要作用,环境适应,和材料合成，硫酸盐转运蛋白是硫代谢的开始。我们在蓝莓基因组中鉴定出37个潜在的VcSULTR基因，编码534到766个氨基酸的肽。在进化分析中，将这些基因分为四个亚家族。37种推定的VcSULTR蛋白的大小范围为60.03至83.87kDa。这些蛋白质被预测为疏水性的并且大部分定位于质膜。VcSULTR基因分布在30条染色体上；VcSULTR3；5b和VcSULTR3；5c是唯一串联重复的基因。VcSULTR启动子含有与真菌共生和应激反应相关的顺式作用元件。VcSULTR的转录水平在蓝莓器官之间有所不同，并且响应于类固醇菌根真菌和硫酸盐处理而发生变化。亚细胞定位分析表明，VcSULTR2;1c定位于，并在中运行，质膜和叶绿体。病毒诱导的VcSULTR2的基因敲低;1c导致内源性硫酸盐含量显着降低，以及编码硫代谢关键酶的基因(VcATPS2和VcSiR1)的上调。这些发现增强了我们对蓝莓中菌根-真菌介导的硫酸盐转运的理解，为蓝莓-菌根共生的进一步研究奠定基础。
