PDF(Pubmed)
Abstract：
The immunogenicity of allogeneic skin fibroblasts in transplantation has been controversial. Whether this controversy comes from a natural heterogeneity among fibroblast subsets or species-specific differences between human and mouse remains to be addressed. In this study, we sought to investigate whether fibroblasts derived from either adult or neonatal human skin tissues could induce different immune responses toward phagocytosis and T cell activation using in vitro co-culture models. Our results indicate that both phagocytosis and T cell proliferation are reduced in the presence of neonatal skin fibroblasts compared to adult skin fibroblasts. We also show that neonatal skin fibroblasts secrete paracrine factors that are responsible for reduced T cell proliferation. In addition, we show that neonatal skin fibroblasts express less class II human leukocyte antigen (HLA) molecules than adult skin fibroblasts after interferon gamma priming, which might also contribute to reduced T cell proliferation. In conclusion, this study supports the use of allogeneic neonatal skin fibroblasts as a readily available cell source for tissue production and transplantation to treat patients with severe injuries.
摘要：
同种异体皮肤成纤维细胞在移植中的免疫原性一直存在争议。这种争议是否来自成纤维细胞亚群之间的自然异质性或人类和小鼠之间的物种特异性差异仍有待解决。在这项研究中,我们试图使用体外共培养模型研究来自成人或新生儿皮肤组织的成纤维细胞是否可以诱导针对吞噬作用和T细胞活化的不同免疫应答.我们的结果表明，与成人皮肤成纤维细胞相比，在新生儿皮肤成纤维细胞的存在下，吞噬作用和T细胞增殖均降低。我们还表明，新生儿皮肤成纤维细胞分泌旁分泌因子，这些因子负责减少T细胞增殖。此外,我们显示，新生儿皮肤成纤维细胞表达较少的II类人类白细胞抗原（HLA）分子比成人皮肤成纤维细胞干扰素γ启动后，这也可能导致T细胞增殖减少。总之,这项研究支持使用同种异体新生儿皮肤成纤维细胞作为组织生产和移植治疗严重损伤患者的现成细胞来源。
