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Abstract：
The Goldblatt model of hypertension (2K-1C) in rats is characterized by renal sympathetic nerve activity (rSNA). We investigated the effects of unilateral renal denervation of the clipped kidney (DNX) on sodium transporters of the unclipped kidneys and the cardiovascular, autonomic, and renal functions in 2K-1C and control (CTR) rats. The mean arterial pressure (MAP) and rSNA were evaluated in experimental groups. Kidney function and NHE3, NCC, ENaCβ, and ENaCγ protein expressions were assessed. The glomerular filtration rate (GRF) and renal plasma flow were not changed by DNX, but the urinary (CTR: 0.0042 ± 0.001; 2K-1C: 0.014 ± 0.003; DNX: 0.005 ± 0.0013 mL/min/g renal tissue) and filtration fractions (CTR: 0.29 ± 0.02; 2K-1C: 0.51 ± 0.06; DNX: 0.28 ± 0.04 mL/min/g renal tissue) were normalized. The Na+/H+ exchanger (NHE3) was reduced in 2K-1C, and DNX normalized NHE3 (CTR: 100 ± 6; 2K-1C: 44 ± 14, DNX: 84 ± 13%). Conversely, the Na+/Cl- cotransporter (NCC) was increased in 2K-1C and was reduced by DNX (CTR: 94 ± 6; 2K-1C: 144 ± 8; DNX: 60 ± 15%). In conclusion, DNX in Goldblatt rats reduced blood pressure and proteinuria independently of GRF with a distinct regulation of NHE3 and NCC in unclipped kidneys.
摘要：
大鼠高血压Goldblatt模型(2K-1C)的特征在于肾交感神经活动(rSNA)。我们研究了单侧肾脏去神经支配的肾脏（DNX）对未夹肾脏和心血管的钠转运蛋白的影响，自主性,2K-1C和对照（CTR）大鼠的肾功能。在实验组中评估平均动脉压（MAP）和rSNA。肾功能和NHE3，NCC，ENaCβ,评估ENaCγ蛋白表达。DNX未改变肾小球滤过率(GRF)和肾血浆流量,但尿液（CTR：0.0042±0.001；2K-1C：0.014±0.003；DNX：0.005±0.0013mL/min/g肾组织）和滤过分数（CTR：0.29±0.02；2K-1C：0.51±0.06；DNX：0.28±0.04mL/min/g肾组织）均归一化。Na+/H+交换剂(NHE3)在2K-1C中还原,和DNX归一化的NHE3(CTR:100±6;2K-1C:44±14,DNX:84±13%)。相反，在2K-1C中Na/Cl-共转运蛋白（NCC）增加，而DNX降低（CTR：94±6；2K-1C：144±8；DNX：60±15％）。总之,Goldblatt大鼠中的DNX独立于GRF降低血压和蛋白尿，并在未修剪的肾脏中明显调节NHE3和NCC。
