PDF(Pubmed)
Abstract：
IL-17A drives inflammation and oxidative stress, affecting the progression of chronic lung diseases (asthma, chronic obstructive pulmonary disease (COPD), lung cancer, and cystic fibrosis). Oleuropein (OLP) is a polyphenolic compound present in olive oil and widely included in the Mediterranean diet. It exerts antioxidant and anti-inflammatory activities, oxidative stress resistance, and anticarcinogenic effects with a conceivable positive impact on human health. We hypothesized that OLP positively affects the mechanisms of oxidative stress, apoptosis, DNA damage, cell viability during proliferation, and cell growth in alveolar epithelial cells and tested its effect in a human alveolar epithelial cell line (A549) in the presence of IL-17A. Our results show that OLP decreases the levels of oxidative stress (Reactive Oxygen Species, Mitochondrial membrane potential) and DNA damage (H2AX phosphorylation-ser139, Olive Tail Moment data) and increases cell apoptosis in A549 cells exposed to IL-17A. Furthermore, OLP decreases the number of viable cells during proliferation, the migratory potential (Scratch test), and the single cell capacity to grow within colonies as a cancer phenotype in A549 cells exposed to IL-17A. In conclusion, we suggest that OLP might be useful to protect lung epithelial cells from oxidative stress, DNA damage, cell growth, and cell apoptosis. This effect might be exerted in lung diseases by the downregulation of IL-17A activities. Our results suggest a positive effect of the components of olive oil on human lung health.
摘要：
IL-17A驱动炎症和氧化应激，影响慢性肺部疾病的进展(哮喘，慢性阻塞性肺疾病(COPD),肺癌,和囊性纤维化）。橄榄苦苷（OLP）是一种存在于橄榄油中的多酚化合物，广泛包含在地中海饮食中。它具有抗氧化和抗炎活性，抗氧化应激，和抗癌作用，对人类健康具有可想而知的积极影响。我们假设OLP积极影响氧化应激的机制，凋亡，DNA损伤,增殖过程中的细胞活力，和肺泡上皮细胞中的细胞生长，并在IL-17A存在下测试了其在人肺泡上皮细胞系（A549）中的作用。我们的结果表明，OLP降低了氧化应激(活性氧，线粒体膜电位）和DNA损伤（H2AX磷酸化-ser139，OliveTailMoment数据）并增加暴露于IL-17A的A549细胞的细胞凋亡。此外，OLP在增殖过程中减少活细胞的数量，迁移潜力(划痕测试)，以及在暴露于IL-17A的A549细胞中作为癌症表型在集落内生长的单细胞能力。总之,我们认为OLP可能有助于保护肺上皮细胞免受氧化应激，DNA损伤,细胞生长,和细胞凋亡。这种作用可能通过下调IL-17A活性而在肺部疾病中发挥。我们的结果表明，橄榄油成分对人体肺部健康有积极影响。
