PDF(Pubmed)
Abstract：
Globally, cognitive impairment (CI) is the leading cause of disability and dependency among the elderly, presenting a significant public health concern. However, there is currently a deficiency in pharmacological interventions that can effectively cure or significantly reverse the progression of cognitive impairment. Methyl donor nutrients (MDNs), including folic acid, choline, and vitamin B12, have been identified as potential enhancers of cognitive function. Nevertheless, there remains a dearth of comprehensive research investigating the connection between the dietary intake of MDNs and CI. In our study, we comprehensively assessed the relationship between MDNs\' dietary intake and CI in older adults, utilizing 16S rRNA gene sequencing to investigate the potential underlying mechanisms. The results showed an obvious difference in the methyl-donor nutritional quality index (MNQI) between the dementia (D) group and the dementia-free (DF) group. Specifically, there was a lower MNQI in the D group than that in the DF group. For the gut microbiome, the beta diversity of gut flora exhibited higher levels in the high methyl-donor nutritional quality (HQ) group as opposed to the low methyl-donor nutritional quality (LQ) group, and lower levels in the D group in comparison to the DF group. Subsequently, we performed a correlation analysis to examine the relationship between the relative abundance of microbiota, the intake of MDNs, and Montreal Cognitive Assessment (MoCA) scores, ultimately identifying ten genera with potential regulatory functions. Additionally, KEGG pathway analyses suggested that the one-carbon metabolism, chronic inflammation, and DNA synthesis potentially serve as pathways through which MDNs may be promising for influencing cognitive function. These results implied that MDNs might have the potential to enhance cognitive function through the regulation of microbiota homeostasis. This study offers dietary recommendations for the prevention and management of CI in the elderly.
摘要：
全球范围内,认知障碍（CI）是老年人残疾和依赖的主要原因，提出了重大的公共卫生问题。然而,目前,药物干预措施缺乏能有效治愈或显著逆转认知障碍的进展.甲基供体营养素（MDN），包括叶酸,胆碱，和维生素B12，已被确定为认知功能的潜在增强剂。然而，关于饮食中MDN摄入与CI之间关系的综合研究仍然缺乏。在我们的研究中,我们全面评估了老年人的饮食摄入量与CI之间的关系，利用16SrRNA基因测序研究潜在的潜在机制。结果表明，痴呆（D）组与无痴呆（DF）组之间的甲基供体营养质量指数（MNQI）存在明显差异。具体来说,D组MNQI低于DF组。对于肠道微生物组，与低甲基供体营养质量（LQ）组相比，高甲基供体营养质量（HQ）组的肠道菌群β多样性水平较高，与DF组相比，D组的水平较低。随后，我们进行了相关性分析，以检查微生物群的相对丰度之间的关系，MDN的摄入量，和蒙特利尔认知评估（MoCA）得分，最终确定具有潜在调节功能的十个属。此外,KEGG通路分析表明，一碳代谢，慢性炎症，和DNA合成可能是MDN有希望影响认知功能的途径。这些结果表明MDN可能具有通过调节微生物群稳态来增强认知功能的潜力。这项研究为老年人CI的预防和管理提供了饮食建议。
