PDF(Pubmed)
Abstract：
The ketogenic diet (KD) and regular exercise (EX) are both capable of orchestrating circadian metabolism homeostasis during losing weight. However, the combined effects of these two factors on circadian metabolism remain poorly understood. To determine if the combined treatment yields a superimposed physiological phenotype, we measured weight loss, white adipose, the respiratory exchange ratio (RER), heat production, and activity parameters in individual and combined treatment groups. Surprisingly, none of these metrics displayed a cumulative effect when administered in the combined treatment approach. Additionally, we investigated the impact of combination therapy on molecular homeostasis through using high-throughput liver transcriptomic approaches. The results revealed that individual and combined treatments can reprogram the circadian rhythm; yet, the combined group exhibited a minimum quantity of cyclic transcript genes. Noteworthy, the amplitude of 24 h circadian expression genes was not significantly increased in the combination treatment, indicating that the combined approach has non-overlapping effects on maintenance peripheral metabolism homeostasis. This may be due to the liver requiring less ketogenic and gluconeogenic potential during metabolic processes. This research suggests that combined treatment may have adverse effects on the body\'s homeostasis and provide crucial insights for the homeostatic health of athletes or individuals who wish to lose weight.
摘要：
生酮饮食（KD）和定期运动（EX）都能够在减肥过程中协调昼夜节律代谢稳态。然而,这两个因素对昼夜节律代谢的综合影响仍然知之甚少。为了确定联合治疗是否产生叠加的生理表型，我们测量了体重减轻，白色脂肪，呼吸交换比(RER)，产热,和单独和联合治疗组的活动参数。令人惊讶的是,当在联合治疗方法中给药时,这些指标均未显示出累积效应.此外,我们通过使用高通量肝脏转录组学方法研究了联合治疗对分子稳态的影响.结果表明，单独和联合治疗可以重新编程昼夜节律；然而，组合组显示出最小数量的循环转录本基因。值得注意的是,24h昼夜节律表达基因的振幅在联合治疗中没有显着增加，表明联合方法对维持外周代谢稳态具有非重叠作用。这可能是由于肝脏在代谢过程中需要较少的生酮和糖异生潜能。这项研究表明，联合治疗可能对身体的稳态产生不利影响，并为希望减肥的运动员或个人的稳态健康提供了重要的见解。
